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Tempone

Name: Tempone
Brand: TargetMol
SKU: T36506
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T36506Cas No. 2896-70-0

Alias 4-Oxo-tempo

Tempone is an aminooxyalkane that acts as a free radical scavenger and reacts with reactive oxygen species (ROS). Intravenous Tempone reduces mean arterial pressure in spontaneously hypertensive rats.

Tempone

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Purity: 98.18%

Catalog No. T36506Alias 4-Oxo-tempoCas No. 2896-70-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 g	$29	-	In Stock

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.18%

Appearance:Solid

Color:Yellow

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COA

Product Introduction

Bioactivity

Description	Tempone is an aminooxyalkane that acts as a free radical scavenger and reacts with reactive oxygen species (ROS). Intravenous Tempone reduces mean arterial pressure in spontaneously hypertensive rats.
In vivo	Tempone (100 mg/kg i.v. bolus followed by 30 mg/kg i.v. infusion; rats) significantly improved both glomerular and tubular function (serum urea, creatinine, creatinine clearance, and fractional excretion of Na(+)) in a dose-dependent manner and significantly attenuated the reperfusion-injury associated with I/R (urinary N-acetyl-beta-D-glucosaminidase, aspartate aminotransferase, assessment of renal histology). Tempone also markedly reduced the immunohistochemical evidence of the formation of nitrotyrosine and poly(ADP-ribose), indicating a reduction of nitrosative and oxidative stress, respectively. Importantly, in contrast to its in vivo metabolite TEMPOL (which also provided protective effects against renal I/R and oxidative stress of PT cells), Tempone reduced renal dysfunction and injury without causing a significant reduction in blood pressure upon administration. These results suggest, for the first time, that Tempone can reduce the renal dysfunction and injury caused by I/R and the injury caused to PT cells by oxidative stress without producing the adverse cardiovascular effects observed when using other nitroxyl radicals.[2]
Synonyms	4-Oxo-tempo

Chemical Properties

Molecular Weight	170.23
Formula	C₉H₁₆NO₂
Cas No.	2896-70-0
Smiles	CC1(C)CC(=O)CC(C)(C)N1[O]
Relative Density.	1.0710 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (264.35 mM), Sonication is recommended.

PBS (pH 7.2): 10 mg/mL (58.74 mM), Sonication is recommended.

DMF: 30 mg/mL (176.23 mM), Sonication is recommended.

Ethanol: 30 mg/mL (176.23 mM), Sonication is recommended.

Solution Preparation Table

PBS (pH 7.2)/DMF/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.8744 mL	29.3720 mL	58.7441 mL	293.7203 mL
5 mM	1.1749 mL	5.8744 mL	11.7488 mL	58.7441 mL
10 mM	0.5874 mL	2.9372 mL	5.8744 mL	29.3720 mL
20 mM	0.2937 mL	1.4686 mL	2.9372 mL	14.6860 mL
50 mM	0.1175 mL	0.5874 mL	1.1749 mL	5.8744 mL

DMF/Ethanol/DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0587 mL	0.2937 mL	0.5874 mL	2.9372 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc