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SMCC

Name: SMCC
Brand: TargetMol
SKU: T9116
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T9116Cas No. 64987-85-5

Alias N-Succinimidyl 4-(N-maleimidomethyl)cycl

SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker.

SMCC

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Purity: 99.53%

Catalog No. T9116Alias N-Succinimidyl 4-(N-maleimidomethyl)cyclCas No. 64987-85-5

SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$30	In Stock	In Stock
100 mg	$45	In Stock	In Stock
500 mg	$85	-	In Stock
1 g	$125	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.53%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	SMCC (N-Succinimidyl 4-(N-maleimidomethyl)cycl) is a heterobifunctional protein crosslinker.
In vitro	N-Succinimidyl 4-(N-maleimidomethyl)cycl（SMCC) is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.
Cell Research	Branched PEI (25 kDa) was dissolved in PBS buffer to yield a concentration of 1 mg/mL. No-weigh Sulfo-SMCC was dissolved in Milli-Q water with a concentration of 10 mg/mL, then a certain of the prepared no-weigh SulfoSMCC solution was added into 2 mL PEI solution to incubate for 30 min at 37 C. After the PEI-SMCC polymer was formed, TAT solution was added into the polymer at the given molar ratio and incubated for 2 h at 4 C. The concentration of unreacted SMCC and unreacted TAT were removed using Zeba Spin Desalting Columns. The polymer PEI-SMCC-TAT was also characterized and analyzed through FTIR to check if each element was added to the PEI backbone.
Synonyms	N-Succinimidyl 4-(N-maleimidomethyl)cycl

Chemical Properties

Molecular Weight	334.32
Formula	C₁₆H₁₈N₂O₆
Cas No.	64987-85-5
Smiles	O=C(ON1C(=O)CCC1=O)C1CCC(CN2C(=O)C=CC2=O)CC1
Relative Density.	1.42 g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 72.14 mg/mL (215.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.98 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9911 mL	14.9557 mL	29.9115 mL	149.5573 mL
5 mM	0.5982 mL	2.9911 mL	5.9823 mL	29.9115 mL
10 mM	0.2991 mL	1.4956 mL	2.9911 mL	14.9557 mL
20 mM	0.1496 mL	0.7478 mL	1.4956 mL	7.4779 mL
50 mM	0.0598 mL	0.2991 mL	0.5982 mL	2.9911 mL
100 mM	0.0299 mL	0.1496 mL	0.2991 mL	1.4956 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc