Shopping Cart

Your shopping cart is currently empty

ML224

Name: ML224
Brand: TargetMol
SKU: T16106
Price: 52 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T16106Cas No. 1338824-21-7

Alias NCGC00242364, ANTAG3

ML224 (NCGC00242364) is a TSHR antagonist for the treatment of Graves' orbital disease and Graves' hyperthyroidism.

ML224

🥰Excellent

Purity: 99.14%

Catalog No. T16106Alias NCGC00242364, ANTAG3Cas No. 1338824-21-7

ML224 (NCGC00242364) is a TSHR antagonist for the treatment of Graves' orbital disease and Graves' hyperthyroidism.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$52	-	In Stock
5 mg	$123	-	In Stock
10 mg	$197	-	In Stock
1 mL x 10 mM (in DMSO)	$142	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.14%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	ML224 (NCGC00242364) is a TSHR antagonist for the treatment of Graves' orbital disease and Graves' hyperthyroidism.
Targets&IC50	FSHR:＞30 μM, LHR:＞30 μM, TSHR:2.1 μM, HEK293 cells:2.1 μM
In vitro	In human embryonic kidney 293 cells, ML224 exhibits a half-maximal inhibitory concentration (IC50) of 2.1 μM against TSHR after a 20-minute exposure, with a range of 0.001-100 μM. The half-maximal inhibitory concentrations against LH and FSH receptors exceed 30 μM[1].
In vivo	ML224(ANTAG3) 2 mg per mouse; administered through intraperitoneal infusion pump; once daily for 3 days) reduces serum FT4 levels and thyroid mRNA of TPO and NIS in mice subjected to prolonged TRH stimulation[1].
Synonyms	NCGC00242364, ANTAG3

Chemical Properties

Molecular Weight	525.59
Formula	C₃₁H₃₁N₃O₅
Cas No.	1338824-21-7
Smiles	COc1ccc(cc1COc1c(C)cc(NC(C)=O)cc1C)C1Nc2ccccc2C(=O)N1Cc1ccco1
Relative Density.	1.252 g/cm3 (Predicted)
Color	Red
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (152.21 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9026 mL	9.5131 mL	19.0262 mL	95.1312 mL
5 mM	0.3805 mL	1.9026 mL	3.8052 mL	19.0262 mL
10 mM	0.1903 mL	0.9513 mL	1.9026 mL	9.5131 mL
20 mM	0.0951 mL	0.4757 mL	0.9513 mL	4.7566 mL
50 mM	0.0381 mL	0.1903 mL	0.3805 mL	1.9026 mL
100 mM	0.0190 mL	0.0951 mL	0.1903 mL	0.9513 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc