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MFI8

Name: MFI8
Brand: TargetMol
SKU: T60503
Price: 71 USD
Availability: InStock
Rating: 5 (10 reviews)

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Purity: 99.95%

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MFI8 is a compound that regulates mitochondrial fission and can be used to study aging.

Cas No. 694488-83-0

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Pack Size	Price	USA Stock	Global Stock
1 mg	$71	In Stock	In Stock
5 mg	$170	In Stock	In Stock
10 mg	$281	In Stock	In Stock
25 mg	$462	In Stock	In Stock
50 mg	$630	In Stock	In Stock
100 mg	$822	-	In Stock
1 mL x 10 mM (in DMSO)	$158	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.95%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	MFI8 is a compound that regulates mitochondrial fission and can be used to study aging.
Targets & IC50	mitochondrial:4.8 μM (EC50)
In vitro	At a concentration of 20 μM for a duration of 6 hours, MFI8 significantly reduces mitochondrial aspect ratio with an EC50 of 4.8 μM. It achieves this by inhibiting mitochondrial function through mitofusins, subsequently promoting mitochondrial fission [1]. MFI8 possesses functional groups that adhere to the pharmacophore model standards. It interacts with mitofusins, facilitating mitochondrial fission [1]. Binding to the HR2 domain of MFN2, MFI8 modulates MFN conformation and complexes, thereby altering mitochondrial function [1].In a concentration-responsive manner (0-20 μM; 6 hours), MFI8 increases caspase-3/7 activity, induces cytochrome c release, and reduces membrane potential in a mitotic protein-dependent manner. Additionally, the combination of MFI8 with BV6 SMAC, a mimic of the second mitochondrial-derived activator of caspases, induces DNA damage and cell death [1].

Chemical Properties

Molecular Weight	275.77
Formula	C₁₆H₁₈ClNO
Cas No.	694488-83-0
Smiles	CC(Nc1cccc(C)c1C)c1cc(Cl)ccc1O

Storage & Solubility Information

Storage

Store at low temperature,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (543.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: < 10 mg/mL (36.26 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: < 10 mg/mL (36.26 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% Corn oil: 10 mg/mL (36.26 mM), Solution.

10% DMSO+90% (20% SBE-β-CD in Saline): < 10 mg/mL (36.26 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6262 mL	18.1311 mL	36.2621 mL	181.3105 mL
5 mM	0.7252 mL	3.6262 mL	7.2524 mL	36.2621 mL
10 mM	0.3626 mL	1.8131 mL	3.6262 mL	18.1311 mL
20 mM	0.1813 mL	0.9066 mL	1.8131 mL	9.0655 mL
50 mM	0.0725 mL	0.3626 mL	0.7252 mL	3.6262 mL
100 mM	0.0363 mL	0.1813 mL	0.3626 mL	1.8131 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc