L 012 sodium salt, a luminol analogue, is a widely used chemiluminescent (CL) probe for active oxygen and active nitrogen. L 012 sodium salt is widely used to detect the superoxides (O2•−) derived from NADPH oxidase (Nox) to find Nox inhibitors.

L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase

In the human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [2].
In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulfate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [3].