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L 012 sodium salt

Catalog No. T22096 CAS 143556-24-5

L 012 sodium salt, a luminol analogue, is a widely used chemiluminescent (CL) probe for active oxygen and active nitrogen. L 012 sodium salt is widely used to detect the superoxides (O2•−) derived from NADPH oxidase (Nox) to find Nox inhibitors.

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L 012 sodium salt, CAS 143556-24-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 51.00
5 mg	In stock	$ 122.00
10 mg	In stock	$ 189.00
25 mg	In stock	$ 323.00
50 mg	In stock	$ 479.00
1 mL * 10 mM (in DMSO)	In stock	$ 133.00

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Purity: 98.08%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	L 012 sodium salt, a luminol analogue, is a widely used chemiluminescent (CL) probe for active oxygen and active nitrogen. L 012 sodium salt is widely used to detect the superoxides (O2•−) derived from NADPH oxidase (Nox) to find Nox inhibitors.
In vitro	L 012 sodium salt is a new chemiluminescent probe. In activated EoL-1 cells which generated reactive oxygen species (ROS), treatment with L-012 generated a significant chemiluminescence. While, superoxide dismutase (100-300 U/ml) abolished the chemiluminescence, which suggested that the main ROS detected was O2- [1]. In cultured endothelial cells, phorbol 12-myristate 13-acetate (PMA, 1 μM) caused an 2.8-fold increase of L-012 chemiluminescence. Vanadate (100 μM) increased the chemiluminescence up to 17-fold. These results suggested that the increase of superoxide produced by NAD(P)H oxidase
In vivo	In the human oral cavity and blood and rat peritoneal cavity, L-012 reacted with ROS generated by activated neutrophils and generated strong chemiluminescence, which was higher than that of MCLA [2]. In nonobese diabetic mice and K8 knockout (K8(-/-)) mice, treatment with dextran sulfate sodium generated an increased L-012 chemiluminescence which detected reactive oxygen and nitrogen species (ROS and RNS) [3].

Molecular Weight	310.67
Formula	C13H8ClN4NaO2
CAS No.	143556-24-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 3.0 mg/mL (9.6 mM), Sonication and heating to 60℃ are recommended.

DMSO: 45.0 mg/mL (144.8 mM), Sonication is recommended.

References and Literature

1. Sohn HY, et al. Sensitive superoxide detection in vascular cells by the new chemiluminescence dye L-012. J Vasc Res. 1999;36(6):456-464. 2. Imada I, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-01Anal Biochem. 1999;271(1):53-58. 3. Asghar MN, et al. In vivo imaging of reactive oxygen and nitrogen species in murine colitis. Inflamm Bowel Dis. 2014;20(8):1435-1447.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

L 012 sodium salt 143556-24-5 L 012 sodium inhibitor inhibit

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