Shopping Cart

Your shopping cart is currently empty

JSH-150

Name: JSH-150
Brand: TargetMol
SKU: T8484
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T8484Cas No. 2247481-21-4

JSH-150 is a highly selective CDK9 inhibitor with an IC50 of 1 nM.

JSH-150

Copy Product Info

😃Good

Purity: 99.96%

Catalog No. T8484Cas No. 2247481-21-4

JSH-150 is a highly selective CDK9 inhibitor with an IC50 of 1 nM.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
5 mg	$89	In Stock	In Stock
10 mg	$156	In Stock	In Stock
25 mg	$347	In Stock	In Stock
50 mg	$538	In Stock	In Stock
100 mg	$651	-	In Stock
500 mg	$1,390	Inquiry	Inquiry

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.96%

Appearance:Solid

Color:White to Red

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	JSH-150 is a highly selective CDK9 inhibitor with an IC50 of 1 nM.
Targets&IC50	CDK7-CyclinH-MNAT1:1.72 μM, CDK9-CyclinT1:1 nM, CDK14-CyclinY:1.68 μM, CDK16-CyclinY:292 nM, CDK1-CyclinB:1.34 μM, CDK5-p25:4.64 μM, CDK2-CyclinA:2.86 μM
In vitro	A highly selective inhibitor compound 40 (JSH-150), which exhibited an IC50 of 1 nM against CDK9 kinase in the biochemical assay and achieved around 300 10000-fold selectivity over other CDK kinase family members.?In addition, it also displayed high selectivity over other 468 kinases/mutants (KINOMEscan S score(1) = 0.01).Compound 40 displayed potent antiproliferative effects against melanoma, neuroblastoma, hepatoma, colon cancer, lung cancer as well as leukemia cell lines.?It could dose-dependently inhibit the phosphorylation of RNA Pol II, suppress the expression of MCL-1 and c-Myc, arrest the cell cycle and induce the apoptosis in the leukemia cells.
In vivo	In the MV4-11 cell-inoculated xenograft mouse model, 10 mg/kg dosage of 40 could almost completely suppress the tumor progression.?The high selectivity and good in vivo PK/PD profile suggested that 40 would be a good pharmacological tool to study CDK9-mediated physiology and pathology as well as a potential drug candidate for leukemia and other cancers.

Chemical Properties

Molecular Weight	505.08
Formula	C₂₄H₃₃ClN₆O₂S
Cas No.	2247481-21-4
Smiles	COCCN[C@H]1CC[C@@H](CC1)Nc1cc(-c2csc(NCC3(CCOCC3)C#N)n2)c(Cl)cn1
Relative Density.	1.29 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (494.97 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.96 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9799 mL	9.8994 mL	19.7988 mL	98.9942 mL
5 mM	0.3960 mL	1.9799 mL	3.9598 mL	19.7988 mL
10 mM	0.1980 mL	0.9899 mL	1.9799 mL	9.8994 mL
20 mM	0.0990 mL	0.4950 mL	0.9899 mL	4.9497 mL
50 mM	0.0396 mL	0.1980 mL	0.3960 mL	1.9799 mL
100 mM	0.0198 mL	0.0990 mL	0.1980 mL	0.9899 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc