DK7575 is a fluorescent dye, a candidate for biological imaging applications, with an appreciable two-photon absorption cross section and strong red light emission.

Instructions
I. Solution preparation:
1. Stock solution: Dissolve DK7575 in an appropriate organic solvent (such as DMSO) to prepare a high concentration stock solution (such as 1 mM).
2. Working solution: Dilute the stock solution to the required working concentration using a physiological buffer (such as PBS).
II. Operation steps
1. Cell staining:
1) Cell preparation: Culture cells and wash with serum-free medium or PBS to remove residual substances that may interfere with staining.
2) Staining: Add DK7575 working solution (1-10uM) to the cell culture medium and mix gently.
3) Incubation: Incubate at an appropriate temperature (such as 37°C) for a certain time (such as 30 minutes) to ensure adequate staining.
4) Washing: Wash cells several times with PBS to remove unbound dye.
2. Tissue staining:
1) Tissue preparation: Fix and permeabilize tissue sections to improve dye permeability.
2) Staining: Cover the tissue sections with DK7575 working solution and incubate for an appropriate time.
3) Washing: Wash the sections with PBS to remove excess dye.
3. Imaging:
Excitation wavelength: Use an excitation wavelength suitable for DK7575 (e.g. 700-800 nm) for two-photon excitation.
Detection wavelength: Collect red fluorescence emission signals (e.g. 600-650 nm).