Coumarin102 (Exciton 480) is a fluorescent dye.Coumarin102 has weak inhibitory activity against Monoamine oxidase B, MAO-B and MAO-A and can be used to study neurological disorders.

I. MAO activity monitoring
1. Material preparation:
(1) Coumarin102 dye: It is recommended to dissolve in DMSO or ethanol with a final concentration of 1-10 μM.
(2) Enzyme source: MAO-B and MAO-A can be extracted from tissues, or commercially purified MAO enzymes can be used.
(3) Fluorescence spectrometer: The excitation wavelength is 365-400 nm, and the emission wavelength is in the range of 460-500 nm.
2. Experimental steps:
(1) Prepare a reaction system containing Coumarin102 and add a specific concentration of MAO-B or MAO-A.
(2) Add substrates (such as phenylethylamine or tyramine) to the reaction system to start the enzyme reaction.
(3) Monitor the changes in fluorescence signals on the fluorescence spectrometer. The interaction between Coumarin102 and substrates or metabolites will cause changes in fluorescence intensity, reflecting MAO activity.
(4) Titration experiments were performed using different concentrations of Coumarin102 to evaluate its inhibitory effect on enzyme activity (IC50 value).
2. Application in the study of nervous system diseases
(1) Cell model study: Using neural cell lines expressing MAO-B or MAO-A (such as SH-SY5Y cells), the intracellular distribution of Coumarin102 and changes in its fluorescence intensity were detected.
(2) Animal model study: In brain tissue sections, the distribution of Coumarin102 was observed by tissue staining and fluorescence microscopy to evaluate its relationship with neuroinflammation or oxidative stress.