Bleomycin hydrochloride is a glycopeptide antibiotic and a DNA synthesis inhibitor. Bleomycin Sulfate can cause DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has anti-tumor activity.

UT-SCC-12B cells:13.0 ± 1.1 nM, UT-SCC-12A cells:14.2 ± 2.8 nM, UT-SCC-19A cells:4.0 ± 1.3 nM

METHODS: Human pancreatic cells (PCCs) AsPC-1 and MIA PaCa-2 were treated with Bleomycin Sulfate (0.1-100 µM) for 24-72 hours, and the cell growth inhibition was detected by the MTT method.
RESULTS: AsPC-1 cells showed no response to Bleomycin Sulfate. Bleomycin Sulfate significantly inhibited the cell growth of MIA-PaCa-2, and the IC50 at 24/48/72 h was 5.9/6.4/2.6 μM, respectively. [1]
METHODS : Mesenchymal stem cells (MSCs) were treated with Bleomycin Sulfate (1800 ng/mL) for 24-96 hours, and sub-G1 and caspase-3 were detected by Flow Cytometry.
RESULTS: Bleomycin Sulfate induces upregulation of sub-G1 and caspase-3 activation, indicating that Bleomycin Sulfate induces apoptosis. [2]
METHODS : Normal rat alveolar macrophages were incubated with Bleomycin Sulfate (0.01-1 μg/mL) for 18 hours, and their levels were determined using the MDGF Assay.
RESULTS: Bleomycin Sulfate stimulates macrophages to produce fibroblast growth factor (MDGF). [3]

METHODS: To detect the anti-tumor activity in vivo, Bleomycin Sulfate (20 mg/kg) was intraperitoneally injected into BALB/c mice carrying mouse colorectal cancer tumor CT26 shControl or shCRT, once every two days for five times.
RESULTS: Bleomycin Sulfate delayed the growth of CT26 cells transfected with control shRNA, but had no effect on CT26 cells transfected with CRT shRNA. [4]
METHODS: To study the time course of Bleomycin induced pulmonary fibrosis in mice, Bleomycin Sulfate (0.06 mg/ mouse in 0.9% saline) was administered as a single intratracheal instillation (IT) to C57Bl/6J mice.
RESULTS: Bleomycin Sulfate induced pulmonary fibrosis in mice. The most appropriate time point for evaluating pulmonary fibrosis in this model is 14 days after IT infusion of Bleomycin Sulfate. This is based on the observation that extensive fibrosis occurred in animals at 14 days, but the variability of the fibrotic response was smaller and the mortality rate was lower than 21 days. [5]