Shopping Cart

Your shopping cart is currently empty

BIX02189

Name: BIX02189
Brand: TargetMol
SKU: T21295
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

😃Good

Catalog No. T21295Cas No. 1265916-41-3

Alias BIX 02189

BIX02189 is a potent and selective inhibitor of MEK5 and ERK5 (IC50: 1.5 nM and 59 nM).

BIX02189

😃Good

Purity: 97.84%

Catalog No. T21295Alias BIX 02189Cas No. 1265916-41-3

BIX02189 is a potent and selective inhibitor of MEK5 and ERK5 (IC50: 1.5 nM and 59 nM).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$29	In Stock	In Stock
5 mg	$46	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$128	In Stock	In Stock
50 mg	$221	In Stock	In Stock
100 mg	$329	In Stock	In Stock
200 mg	$475	-	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:97.84%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	BIX02189 is a potent and selective inhibitor of MEK5 and ERK5 (IC50: 1.5 nM and 59 nM).
Targets&IC50	FGFR1:1 μM, CSF1R (FMS):46 nM, c-Kit:1.1 μM, RPS6KA6/RSK4:990 nM, ERK5:59 nM, RPS6KA3/RSK2:2.1 μM, SRC:7.6 μM, Abl-1:2.4 μM, Lck:250 nM, MEK5:1.5 nM, MAPK14 (p38 alpha):3.7 μM, JAK3:440 nM, TGFβRI:580 nM
In vitro	BIX02189, which inhibited catalytic function of purified, MEK5 enzyme.?The MEK5 inhibitors blocked phosphorylation of ERK5, without affecting phosphorylation of ERK1/2 in sorbitol-stimulated HeLa cells.?The compounds also inhibited transcriptional activation of MEF2C, a downstream substrate of the MEK5/ERK5 signaling cascade, in a cellular trans-reporter assay system[1].
Synonyms	BIX 02189

Chemical Properties

Molecular Weight	440.54
Formula	C₂₇H₂₈N₄O₂
Cas No.	1265916-41-3
Smiles	CN(C)Cc1cccc(N\C(=C2/C(=O)Nc3cc(ccc23)C(=O)N(C)C)c2ccccc2)c1
Relative Density.	1.230 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 49.4 mg/mL (112.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2699 mL	11.3497 mL	22.6994 mL	113.4971 mL
5 mM	0.4540 mL	2.2699 mL	4.5399 mL	22.6994 mL
10 mM	0.2270 mL	1.1350 mL	2.2699 mL	11.3497 mL
20 mM	0.1135 mL	0.5675 mL	1.1350 mL	5.6749 mL
50 mM	0.0454 mL	0.2270 mL	0.4540 mL	2.2699 mL
100 mM	0.0227 mL	0.1135 mL	0.2270 mL	1.1350 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc