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BD750

Name: BD750
Brand: TargetMol
SKU: T8546
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8546Cas No. 892686-59-8

BD750 is an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation(IC50 of 1.5 μM and 1.1 μM in mouse and human T cells, respectively)

BD750

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Purity: 99.02%

Catalog No. T8546Cas No. 892686-59-8

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$38	In Stock	In Stock
25 mg	$78	In Stock	In Stock
50 mg	$123	In Stock	In Stock
100 mg	$186	In Stock	In Stock
200 mg	$275	-	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

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Purity:99.02%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC LCMS

Product Introduction

Bioactivity

Description	BD750 is an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation(IC50 of 1.5 μM and 1.1 μM in mouse and human T cells, respectively)
In vitro	Following BD750 treatment, LPS-induced maturation of DC, allogeneic T cell proliferation, Th1 and Th17 cell functional differentiation, the STAT5 and AKT activation were determined.?The effect of tolDC loaded with antigen peptide on the development and severity of EAE and their splenic Th1 and Th17 cell responses were determined.?In comparison with LPS-induced mature DC (mDC), BD750 treatment induced tolDC with lower expression levels of costimulatory molecules and pro-inflammatory cytokines and lower levels of STAT5 phosphorylation.?TolDC inhibited allogeneic T cell proliferation and reduced Th1 and Th17 responses[1].
Animal Research	Female C57BL/6 mice (10 wks old, 19-21 g).Dosage with 20 μM, and administration:IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15)[1].

Chemical Properties

Molecular Weight	271.34
Formula	C₁₄H₁₃N₃OS
Cas No.	892686-59-8
Smiles	Oc1c2CCCCc2nn1-c1nc2ccccc2s1
Relative Density.	1.54 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 27.5 mg/mL (101.35 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2 mg/mL (7.37 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.6854 mL	18.4271 mL	36.8541 mL	184.2707 mL
5 mM	0.7371 mL	3.6854 mL	7.3708 mL	36.8541 mL
10 mM	0.3685 mL	1.8427 mL	3.6854 mL	18.4271 mL
20 mM	0.1843 mL	0.9214 mL	1.8427 mL	9.2135 mL
50 mM	0.0737 mL	0.3685 mL	0.7371 mL	3.6854 mL
100 mM	0.0369 mL	0.1843 mL	0.3685 mL	1.8427 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc