Anticanceragent 201 (Compound 2f) exhibits IC50 values in the low micromolar range across various tumor cell lines. It demonstrates high in vitro cytotoxicity against CCRF-CEM cells by inducing apoptosis through the activation of caspase-3 in the mitochondrial intrinsic pathway, cleavage of PARP, and reduced expression of Bcl-2 and Bcl-XL proteins. Anticanceragent 201 can be utilized in cancer research.

Anticancer agent 201 (Compound 2f) demonstrates significant cytotoxic effects and induces apoptosis in CCRF-CEM cells, with a dose-dependent decrease in mitochondrial membrane potential at concentrations of 3.5 μM and 17.5 μM over 24 hours. This compound also disrupts or slows the cell cycle in the G0/G1 phase, reducing the proportion of S phase cells and inhibiting RNA synthesis. Additionally, Anticancer agent 201 exhibits cytotoxic activity against eight cancer cell lines, including multidrug-resistant variants, and two normal fibroblast cell lines. The IC50 values (μM) for various cell lines are as follows: CCRF-CEM (3.5), CEM-DNR (4.9), K562 (23), K562-TAX (17), A549 (11), HCT116 (10), HCT116p53 -/- (18), U20S (19), BJ (>50), and MRC-5 (27).