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TAME hydrochloride

Catalog No. T1731 CAS 1784-03-8

Synonyms: Tos-Arg-OMe HCl, NA-P-甲苯磺酰-L-精氨酸甲酯盐酸盐, TOS-ARG-OME hydrochloride

TOS-ARG-OME hydrochloride is a kind od amino acid derivatives.

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TAME hydrochloride, CAS 1784-03-8

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

TOS-ARG-OME hydrochloride is a kind od amino acid derivatives.

Kinase Assay

This clonal cell line, referred to as HEK 293 4.0-7 cells, are used in a high-throughput screening format to detect agonists and allosteric activators of the Ca2+?receptor. Changes in the concentration of cytoplasmic [Ca2+]i provide a quantitative and functional assessment of Ca2+ receptor activity in these cells and the results using this assay parallel those obtained using a homologous expression system of bovine parathyroid cells. On-line continuous measurements of fluorescence in fluo-3- or fura-2-loaded HEK 293 4.0-7 cells are obtained using a custom-built spectrofluorimeter or a fluorescence imaging plate reader instrument. NPS-2143 is incubated with cells for 1 minute before increasing the concentration of extracellular Ca2+?from 1.0 mM to 1.75 mM. NPS-2143 is tested individually at a concentration of 100 μg/mL (20 μM-80 μM) and those causing more than a 40% inhibition of the control response are considered to be biologically active. To determine the potencies (IC50) of NPS-2143 with biological activity, concentration-response curves are obtained and then, as an initial assessment of selectivity, the effects of NPS-2143 on [Ca2+]i evoked by other G protein-coupled receptors are examined at a concentration several times their IC50. Wild-type HEK 293 cells (and HEK 293 4.0-7 cells) express receptors for thrombin, bradykinin, and ATP, which couple to the mobilization of intracellular Ca2+. These responses can be studied to quickly assess any nonselective action of compounds on G protein-coupled receptors. Additional assays for selectivity include HEK 293 cells engineered to express receptors most homologous in sequence and topology to the Ca2+ receptor. These include native or chimeric receptors for various metabotropic glutamate and γ-aminobutyric acid type B receptors (GABABRs).Chimeric receptors are created using partial sequences of metabotropic glutamate receptors and Ca2+?receptors, engineered to couple to activation of phospholipase C and release of intracellular Ca2+?in HEK 293 cells. NPS-2143 lacking pan-activity are then subjected to structural modifications and their potencies and selectivities monitored using these HEK 293 4.0-7 cell assays in an iterative process.

Synonyms	Tos-Arg-OMe HCl, NA-P-甲苯磺酰-L-精氨酸甲酯盐酸盐, TOS-ARG-OME hydrochloride
Molecular Weight	378.87
Formula	C14H23ClN4O4S
CAS No.	1784-03-8

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 37.9 mg/mL(100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Ma Y, et al. Differential effects of Mg(ii) and N(alpha)-4-tosyl-l-arginine methyl ester hydrochloride on the recognition and catalysis in ATP hydrolysis. Dalton Trans. 2008 Feb 28;(8):1081-6. 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26;8(4):383-92. 3. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell. 2010 Oct 19;18(4):382-95.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Inhibitor Library PPI Inhibitor Library Anti-Aging Compound Library Bioactive Compound Library

Related Products

Related compounds with same targets

Boc-L-aspartic acid 4-benzyl ester Boc-L-Glutamic acid 5-benzylester Fmoc-L-Proline Nalpha-Fmoc-L-Glutamine Fmoc-L-glutamic acid 5-tert-butyl ester Fmoc-L-aspartic acid Fmoc-Cys(Acm)-OH Z-Asp(OBzl)-OH

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

TAME hydrochloride 1784-03-8 代谢细胞周期 Amino Acids and Derivatives APC Tos-Arg-OMe HCl inhibit Inhibitor TOS-ARG-OME hydrochloride TAME Anaphase promoting complex inhibitor

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