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TAME

TAME
Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor.
Catalog No. T6693Cas No. 901-47-3
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TAME

Catalog No. T6693Cas No. 901-47-3
Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 mL x 10 mM (in DMSO)$467-10 days
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Product Introduction

Bioactivity
Description
Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor.
Targets&IC50
APC:12 µM.
In vitro
TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 μM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6]
AliasTosylarginine methyl ester, N-4-Tosyl-L-arginine methyl ester
Chemical Properties
Molecular Weight342.41
FormulaC14H22N4O4S
Cas No.901-47-3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
H2O: 63 mg/mL (184 mM)
Ethanol: 3 mg/mL (8.76 mM)
DMSO: 64 mg/mL (186.9 mM)
Solution Preparation Table
Ethanol/H2O/DMSO
1mg5mg10mg50mg
1 mM2.9205 mL14.6024 mL29.2048 mL146.0238 mL
5 mM0.5841 mL2.9205 mL5.8410 mL29.2048 mL
H2O/DMSO
1mg5mg10mg50mg
10 mM0.2920 mL1.4602 mL2.9205 mL14.6024 mL
20 mM0.1460 mL0.7301 mL1.4602 mL7.3012 mL
50 mM0.0584 mL0.2920 mL0.5841 mL2.9205 mL
100 mM0.0292 mL0.1460 mL0.2920 mL1.4602 mL

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