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Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mL x 10 mM (in DMSO) | $46 | 7-10 days |
Description | Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor. |
Targets&IC50 | APC:12 µM. |
In vitro | TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 μM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6] |
Alias | Tosylarginine methyl ester, N-4-Tosyl-L-arginine methyl ester |
Molecular Weight | 342.41 |
Formula | C14H22N4O4S |
Cas No. | 901-47-3 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||||||||||||
Solubility Information | H2O: 63 mg/mL (184 mM) Ethanol: 3 mg/mL (8.76 mM) DMSO: 64 mg/mL (186.9 mM) | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
Ethanol/H2O/DMSO
H2O/DMSO
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