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TAME

Catalog No. T6693   CAS 901-47-3
Synonyms: Tosylarginine methyl ester, N-4-Tosyl-L-arginine methyl ester

Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor.

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TAME Chemical Structure
TAME, CAS 901-47-3
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1 mL * 10 mM (in DMSO) 5 days $ 46.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Tosyl-L-Arginine Methyl Ester (TAME (N-4-Tosyl-L-arginine methyl ester)) is an APC inhibitor.
Targets&IC50 APC:12 µM.
In vitro TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 μM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6]
Synonyms Tosylarginine methyl ester, N-4-Tosyl-L-arginine methyl ester
Molecular Weight 342.41
Formula C14H22N4O4S
CAS No. 901-47-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 63 mg/mL (184 mM)

Ethanol: 3 mg/mL (8.76 mM)

DMSO: 64 mg/mL (186.9 mM)

TargetMolReferences and Literature

1. Zeng X, et al. Cancer Cell, 2010, 18(4), 382-395. 2. Khantaphant S, et al. Food Chem, 2010, 120(3), 658-664. 3. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26;8(4):383-92. 4. Ma Y, et al. Dalton Trans, 2008, 28(8), 1081-1086. 5. Wuytack EY, et al. Appl Environ Microbiol, 2000, 66(1), 257-261.

Related Products

Related compounds with same targets
TASIN-1 Hydrochloride Apcin TASIN-1 TAME hydrochloride Apcin A HCL Activated Protein C (390-404), human acetate CFM 4

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Keywords

TAME 901-47-3 Cell Cycle/Checkpoint APC Inhibitor Anaphase promoting complex Tosylarginine methyl ester N-4-Tosyl-L-arginine methyl ester inhibit inhibitor

 

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