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Streptozocin

Catalog No. T1507   CAS 18883-66-4
Synonyms: STZ, Streptozotocin, NSC-85998, U 9889

Streptozocin (NSC-85998) is an antibiotic that induces DNA methylation. Streptozocin is toxic to insulin-producing pancreatic islet B-cells and is commonly used in the construction of animal models of type 1 diabetes. The product is unstable in solution and is recommended to be dispensed now.

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Streptozocin Chemical Structure
Streptozocin, CAS 18883-66-4
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Purity: 100%
Purity: 99.56%
Purity: 99.32%
Purity: 98.89%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Streptozocin (NSC-85998) is an antibiotic that induces DNA methylation. Streptozocin is toxic to insulin-producing pancreatic islet B-cells and is commonly used in the construction of animal models of type 1 diabetes. The product is unstable in solution and is recommended to be dispensed now.
Targets&IC50 DNA methylating (C1498 cells):1024 μg/mL, DNA methylating (HL60 cells):11.7 μg/mL, DNA methylating (K562 cells):904 μg/mL
In vitro METHODS: Rat β-insulinoma cells Rin-5F were treated with Streptozocin (1-10 mM) for 2-48 h. Cell viability was measured using MTT assay.
RESULTS: Maximum inhibition (60-70%) was observed in cells treated with 10 mM Streptozocin for 24 h and 48 h. The cells were treated with Streptozocin (1-10 mM) for 2-48 h, and cell viability was measured by MTT assay. [1]
METHODS: Neural stem cells were treated with Streptozocin (2.5 mM) for 2 days and gene expression was detected using RT-qPCR.
RESULTS: Streptozocin significantly reduced the relative expression level of GLUT3 mRNA by 46.4%, and did not affect the relative expression level of IR and GLUT1. [2]
In vivo METHODS: Streptozocin (40 mg/kg in the 50 mM sodium citrate buffer (pH 4.5), ready to use) was administered intraperitoneally to C57BL/6 or CD-1 male mice once daily for five days. Normal food and 10% sucrose water were provided during the administration period, and 10% sucrose water was replaced with normal water on the sixth day.
RESULTS: Repeated low doses of Streptozocin induced type 1 diabetes in mice. [3]
METHODS: Streptozocin (200 mg/kg in the 50 mM sodium citrate buffer (pH 4.5), ready to use) was administered to C57BL/6 or CD-1 male mice by a single intraperitoneal injection. Normal food and 10% sucrose water were provided after administration, and 10% sucrose water was replaced with normal water on the third day.
RESULTS: A single high dose of Streptozocin induced type 1 diabetes in mice. [3]
Cell Research Streptozocin (STZ) stock solutions of 50 mg/mL in distilled Water (dWater) are freshly prepared for each experiment[2]. Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dWater in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2].
Synonyms STZ, Streptozotocin, NSC-85998, U 9889
Molecular Weight 265.22
Formula C8H15N3O7
CAS No. 18883-66-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 113.3 mg/mL (427.19 mM), Sonication and heating are recommended.

DMSO: 16.67 mg/mL (62.84 mM), Sonication is recommended.(The compound is unstable in solution, please use soon.)

TargetMolReferences and Literature

1. Nahdi AMTA, et al. Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β-Cells. Oxid Med Cell Longev. 2017;2017:7054272. 2. Sun P, et al. Streptozotocin Impairs Proliferation and Differentiation of Adult Hippocampal Neural Stem Cells in Vitro-Correlation With Alterations in the Expression of Proteins Associated With the Insulin System. Front Aging Neurosci. 2018 May 18;10:145. 3. Furman BL. Streptozotocin-Induced Diabetic Models in Mice and Rats. Curr Protoc Pharmacol. 2015 Sep 1;70:5.47.1-5.47.20. 4. Iwase M, et al. Tohoku J Exp Med, 1989, 159(2), 83-90. 6. Acer S, et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug;79(4):222-5.

TargetMolCitations

1. Li Y, Zhou X, Cheng C, et al.Gut AstA mediates sleep deprivation-induced energy wasting in Drosophila.Cell Discovery.2023, 9(1): 49.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Clinical Compound Library Tyrosine Kinase Inhibitor Library Anti-Cancer Drug Library Anti-Cancer Active Compound Library Microbial Natural Product Library Human Metabolite Library RO5 Drug-like Natural Product Library Natural Product Library for HTS DNA Damage & Repair Compound Library Anti-Cancer Compound Library

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Keywords

Streptozocin 18883-66-4 Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology DNA Alkylator/Crosslinker DNA Alkylation Antibacterial DNA/RNA Synthesis Antibiotic STZ inhibit U9889 Streptozotocin U-9889 Bacterial NSC85998 Inhibitor NSC-85998 NSC 85998 U 9889 inhibitor

 

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