Home Tools

Reversine

Catalog No. T1825 CAS 656820-32-5

Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Reversine, CAS 656820-32-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 32.00
2 mg	In stock	$ 45.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 81.00
25 mg	In stock	$ 167.00
50 mg	In stock	$ 235.00
100 mg	In stock	$ 413.00
1 mL * 10 mM (in DMSO)	In stock	$ 64.00

Bulk Inquiry

Select Batch

Purity: 99.75%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).
Targets&IC50	Aurora A:150-500 nM, Aurora B:150-500 nM, Aurora C:150-500 nM
In vitro	In mice carrying U14 tumors, intraperitoneal injection of Reversine (10 mg/kg) effectively reduces tumor cells.
In vivo	In stably transfected Chinese hamster ovary cells, Reversine can competitively inhibit cAMP production stimulated by. In HCT116 cells, Reversine suppresses the Aurora targets and phosphorylation of histone H3. Additionally, in primary human tumor samples, Reversine is capable of inhibiting the formation of leukemia cell colonies.
Kinase Assay	Radioligand Binding Assays: Each tube in the A3 AR competitive binding assay contains 100 μL of membrane suspension (20 μg of protein), 50 μL of [125I]4-amino-3-iodobenzyl)adenosine-5′-N-methyluronamide (0.5 nM), and 50 μL of increasing concentrations of the test ligands in Tris-HCl buffer (50 mM, pH 7.4) containing 10 mM MgCl2 and 1 mM EDTA. Nonspecific binding is determined using 10 mM 5′-N-ethylcarboxamidoadenosine in the buffer. The mixtures are incubated at 25°C for 60 min. Binding reactions are terminated by filtration through Whatman GF/B filters under reduced pressure using a MT-24 cell harvester. Filters are washed three times with 9 mL of ice-cold buffer. Radioactivity is determined using a Beckman γ-counter, and the percent inhibition is calculated.
Cell Research	Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2 × 104?cells for each well are plated in a 96-well plate in presence of crescent quantity of reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate.(Only for Reference)

Molecular Weight	393.49
Formula	C21H27N7O
CAS No.	656820-32-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 5 mg/mL (12.71 mM), Heating is recommended.

References and Literature

1. Chen S, et al. J Am Chem Soc. 2004, 126(2), 410-411. 2. Perreira M, et al. J Med Chem. 2005, 48(15), 4910-4918. 3. D'Alise AM, et al. Mol Cancer Ther. 2008, 7(5), 1140-1149. 4. Qin HX, et al. Cytotechnology. 2013, 65(4), 643-653. 5. Lima K, Carlos J A E G, de Melo Alves-Paiva R, et al. Reversine exhibits antineoplastic activity in JAK2 V617F-positive myeloproliferative neoplasms[J]. Scientific reports. 2019, 9(1): 9895. 6. Carlos J A E G, Lima K, Coelho-Silva J L, et al. Reversine exerts cytotoxic effects through multiple cell death mechanisms in acute lymphoblastic leukemia[J]. Cellular Oncology. 2020: 1-11.

Citations

1. Carlos J A E G, Lima K, Coelho-Silva J L, et al. Reversine exerts cytotoxic effects through multiple cell death mechanisms in acute lymphoblastic leukemia. Cellular Oncology. 2020: 1-11 2. Lima K, Carlos J A E G, de Melo Alves-Paiva R, et al. Reversine exhibits antineoplastic activity in JAK2 V617F-positive myeloproliferative neoplasms. Scientific Reports. 2019, 9(1): 9895 3. Tan M, Ren F, Yang X. Anti-HBV therapeutic potential of small molecule 3, 5, 6, 7, 3′, 4′-Hexamethoxyflavone in vitro and in vivo. Virology. 2021 4. Hirakata, Camila, et al. Targeting glioma cells by antineoplastic activity of reversine. Oncology Letters. 22.2 (2021): 1-9 5. Hirakata, Camila, et al. Targeting glioma cells by antineoplastic activity of reversine. Oncology Letters. 22.2 (2021): 1-9 6. Parducci N S, Garnique A D M B, Lima K, et al.Antineoplastic effects of pharmacological inhibitors of aurora kinases in CSF3RT618I-driven cells.Blood Cells, Molecules, and Diseases.2023: 102799.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library Membrane Protein-targeted Compound Library Anti-Neurodegenerative Disease Compound Library GPCR Compound Library Neuronal Signaling Compound Library Target-Focused Phenotypic Screening Library Stem Cell Differentiation Compound Library Autophagy Compound Library Bioactive Compounds Library Max

Related Products

Related compounds with same targets

MK-8745 Alisertib VX-11e LY3295668 SCH-1473759 MBM-17S L 888607 Racemate PF-03814735

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Reversine 656820-32-5 Autophagy Cell Cycle/Checkpoint Chromatin/Epigenetic GPCR/G Protein Neuroscience Adenosine Receptor Aurora Kinase inhibit Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.