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Cell Cycle/Checkpoint Aurora Kinase Reversine

Reversine

Catalog No. T1825   CAS 656820-32-5

Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).

Reversine, CAS 656820-32-5
Pack Size Availability Price/USD Quantity
2 mg In stock 50.00
5 mg In stock 72.00
10 mg In stock 90.00
25 mg In stock 186.00
50 mg In stock 262.00
100 mg In stock 460.00
200 mg In stock 828.00
1 mL * 10 mM (in DMSO) In stock 72.00
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Purity 98.00%
Purity 99.75%
Purity 98.00%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Reversine, a small synthetic purine analogue (2, 6-disubstituted purine), is a potent inhibitior of Aurora A/B/C(IC50s=150-500 nM).
Targets&IC50 A3 : 0.66μM(Ki) ,   Aurora A : ic50 12nM ,   Aurora B : ic50 13nM ,   Aurora C : ic50 20nM
Kinase Assay Radioligand Binding Assays: Each tube in the A3 AR competitive binding assay contains 100 μL of membrane suspension (20 μg of protein), 50 μL of [125I]4-amino-3-iodobenzyl)adenosine-5′-N-methyluronamide (0.5 nM), and 50 μL of increasing concentrations of the test ligands in Tris-HCl buffer (50 mM, pH 7.4) containing 10 mM MgCl2 and 1 mM EDTA. Nonspecific binding is determined using 10 mM 5′-N-ethylcarboxamidoadenosine in the buffer. The mixtures are incubated at 25°C for 60 min. Binding reactions are terminated by filtration through Whatman GF/B filters under reduced pressure using a MT-24 cell harvester. Filters are washed three times with 9 mL of ice-cold buffer. Radioactivity is determined using a Beckman γ-counter, and the percent inhibition is calculated.
Cell Research
Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2 × 104 cells for each well are plated in a 96-well plate in presence of crescent quantity of reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate.(Only for Reference)
Cell lines: HL-60, A375, HeLa, HCT-116, T47D, and MCF-7 cell lines
Animal Research
Animal Model: Mice bearing U14 tumors
Molecular Weight 393.49
Formula C21H27N7O
CAS No. 656820-32-5

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 5 mg/mL (12.71 mM), warmed

Ethanol: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Chen S, et al. J Am Chem Soc. 2004, 126(2), 410-411. 2. Perreira M, et al. J Med Chem. 2005, 48(15), 4910-4918. 3. D'Alise AM, et al. Mol Cancer Ther. 2008, 7(5), 1140-1149. 4. Qin HX, et al. Cytotechnology. 2013, 65(4), 643-653. 5. Lima K, Carlos J A E G, de Melo Alves-Paiva R, et al. Reversine exhibits antineoplastic activity in JAK2 V617F-positive myeloproliferative neoplasms[J]. Scientific reports. 2019, 9(1): 9895. 6. Carlos J A E G, Lima K, Coelho-Silva J L, et al. Reversine exerts cytotoxic effects through multiple cell death mechanisms in acute lymphoblastic leukemia[J]. Cellular Oncology. 2020: 1-11.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Stem cell Differentiation Compound Library Autophagy Compound Library Neuronal Signaling Compound Library Epigenetics Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library GPCR Compound Library Anti-cancer Clinical Compound Library Cell cycle related Compound Library

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