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Nexturastat A

Nexturastat A
Nexturastat A is an effective and specific HDAC6 inhibitor (IC50: 5 nM). Its selectivity is >190-fold than other HDACs.
Catalog No. T1819Cas No. 1403783-31-2
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Purity:99.57%
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Nexturastat A

Catalog No. T1819Cas No. 1403783-31-2
Nexturastat A is an effective and specific HDAC6 inhibitor (IC50: 5 nM). Its selectivity is >190-fold than other HDACs.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 mg$63In Stock
5 mg$90In Stock
10 mg$117In Stock
25 mg$197In Stock
50 mg$318In Stock
100 mg$473In Stock
1 mL x 10 mM (in DMSO)$71In Stock
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Product Introduction

Bioactivity
Description
Nexturastat A is an effective and specific HDAC6 inhibitor (IC50: 5 nM). Its selectivity is >190-fold than other HDACs.
Targets&IC50
HDAC6:5 nM
In vivo
Nexturastat A significantly inhibits the growth of B16 melanoma cells in mice, with a GI50 of 14.3 μM. In these cells, Nexturastat A dose-dependently increases the levels of acetylated α-tubulin without affecting the acetylation of histone H3 associated with abscesses.
Kinase Assay
HDAC inhibition assays: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation.
Cell Research
B16 murine melanoma cells are plated at 5000/well in 96 well flat bottom plates. The following day, media is changed to that containing various concentrations of HDACi or matched DMSO vehicle concentrations diluted in complete medium done in triplicate. Cells are incubated for 48 hours at 37°C and 5% CO2. Density of viable, metabolically active cells is quantified using a standard MTS assay as per manufacturer’s instructions. Briefly, 20μL of reagent are added per well and incubated at 37°C for 3 hours. Absorbances at 490 nM are measured spectrophotometrically with background subtraction at 690 nM. All values are then normalized and expressed as a percentage of medium control (100%). (Only for Reference)
Chemical Properties
Molecular Weight341.4
FormulaC19H23N3O3
Cas No.1403783-31-2
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: 2 mg/mL (5.85 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 63 mg/mL (184.5 mM)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.9291 mL14.6456 mL29.2912 mL146.4558 mL
5 mM0.5858 mL2.9291 mL5.8582 mL29.2912 mL
DMSO
1mg5mg10mg50mg
10 mM0.2929 mL1.4646 mL2.9291 mL14.6456 mL
20 mM0.1465 mL0.7323 mL1.4646 mL7.3228 mL
50 mM0.0586 mL0.2929 mL0.5858 mL2.9291 mL
100 mM0.0293 mL0.1465 mL0.2929 mL1.4646 mL

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