Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MRT68921 is a potent and dual autophagy kinase ULK1/2 inhibitor with IC50 of 2.9 nM and 1.1 nM, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 35.00 | |
5 mg | In stock | $ 57.00 | |
10 mg | In stock | $ 97.00 | |
25 mg | In stock | $ 197.00 | |
50 mg | In stock | $ 357.00 | |
100 mg | In stock | $ 449.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 61.00 |
Description | MRT68921 is a potent and dual autophagy kinase ULK1/2 inhibitor with IC50 of 2.9 nM and 1.1 nM, respectively. |
Targets&IC50 | ULK2:1.1 nM, ULK1:2.9 nM |
In vitro | MRT68921 specifically disrupts autophagosome maturation and thus blocks autophagic flux through ULK1 inhibition. [1] |
Kinase Assay | Kinase assays are carried out in 50 mM Tris-HCl, pH 7.4, 10 mM magnesium acetate, 0.1 mM EGTA, and 0.1% β-mercaptoethanol, containing 30 μM cold ATP, and 0.5 μCi of [γ-32P]ATP for 5 min at 25 °C. Prior to ATP addition, reaction mixes are pre-warmed to 25 °C for 5 min. Reactions are stopped by the addition of sample buffer, followed by SDS-PAGE, transfer to nitrocellulose, and analysis by autoradiography and immunoblot[1]. |
Cell Research | MEFs and 293T cells are grown in DMEM, supplemented with 10% fetal bovine serum and penicillin/streptomycin, and cultured at 37°C, 5% CO2. For induction of autophagy, cells are typically grown to 75% confluency, ished twice, and incubated in Earle's balanced salt solution (EBSS) for 1 h (or complete medium as a control). MRT67307 (10 μM), MRT68921 (1 μM), AZD8055 (1 μM), or bafilomycin A1 (50 nM) is included[1]. |
Synonyms | MRT68921 HCl |
Molecular Weight | 434.58 |
Formula | C25H34N6O |
CAS No. | 1190379-70-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 10 mg/mL, warming
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
MRT68921 1190379-70-4 Autophagy ULK Unc-51 like kinase MRT-68921 MRT68921 HCl Inhibitor MRT 68921 inhibit inhibitor