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Lactacystin

Catalog No. T15702 CAS 133343-34-7

Lactacystin is an Antibiotic Streptomyces spp. the metabolite and is an effective and selective Proteasome inhibitor (IC50: 4.8 μM for 20S Proteasome). Lactacystin inhibits cell growth and induces neurite outgrowth and also inhibits the lysosomal enzyme cathepsin A.

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Lactacystin, CAS 133343-34-7

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Lactacystin is an antibiotic Streptomyces spp. the metabolite and is an effective and selective proteasome inhibitor (IC50: 4.8 μM for 20S proteasome). Lactacystin inhibits cell growth and induces neurite outgrowth and also inhibits the lysosomal enzyme cathepsin A.
Targets&IC50	Proteasome:4.8 μM
In vivo	After injection in young and adult mice, Lactacystin (microinjection; 2 μg) causes a Parkinson's disease-like motor phenotype 5-7 days [2].

Molecular Weight	376.43
Formula	C15H24N2O7S
CAS No.	133343-34-7

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Csizmadia V, et al. Effect of proteasome inhibitors with different chemical structures on the ubiquitin-proteasomesystem in vitro. Vet Pathol. 2010 Mar;47(2):358-67. 2. Savolainen MH, et al. Nigral injection of a proteasomal inhibitor, lactacystin, induces widespread glial cell activationand shows various phenotypes of Parkinson's disease in young and adult mouse. Exp Brain Res. 2017 Jul;235(7):2189-2202.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Lactacystin 133343-34-7 Proteases/Proteasome Ubiquitination Proteasome Inhibitor inhibitor inhibit

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