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Hoechst 33258 analog 3

Catalog No. T19005 CAS 23554-98-5

Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA.

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Hoechst 33258 analog 3 Chemical Structure

Hoechst 33258 analog 3, CAS 23554-98-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 163.00
5 mg	In stock	$ 347.00
10 mg	In stock	$ 497.00
25 mg	In stock	$ 793.00
50 mg	In stock	$ 1,130.00
100 mg	In stock	$ 1,520.00
500 mg	In stock	$ 2,970.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA.

In vitro

The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around an emission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage, the solutions are instead frozen at ≤-20 °C. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells.

Molecular Weight	422.52
Formula	C26H26N6
CAS No.	23554-98-5

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 3.57 mg/mL (8.45 mM), Sonication and heating to 70℃ are recommended.

References and Literature

1. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. 2. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. 3. a b c "Hoechst Stains". Invitrogren (Molecular Probes).

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

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Hoechst 33258 analog 3 23554-98-5 Others Inhibitor inhibit inhibitor

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