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Coelenterazine

Catalog No. T6808 CAS 55779-48-1

Synonyms: Oplophorus luciferin, Coelenteramine

Coelenterazine (Coelenteramine) is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques.

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Coelenterazine, CAS 55779-48-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 97.00
2 mg	In stock	$ 148.00
5 mg	In stock	$ 319.00
10 mg	In stock	$ 558.00
25 mg	In stock	$ 721.00
50 mg	In stock	$ 987.00
100 mg	In stock	$ 1,370.00

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Purity: 99.02%

Purity: 98.5%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Coelenterazine (Coelenteramine) is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques.
In vitro	When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2]
In vivo	In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology. [2]
Cell Research	Cells are plated at a density of 5×10^4?cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2?and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only.
Animal Research	Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only.

Synonyms	Oplophorus luciferin, Coelenteramine
Molecular Weight	423.46
Formula	C26H21N3O3
CAS No.	55779-48-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 78 mg/mL (184.2 mM)

Ethanol: 1 mg/mL (2.36 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Morse D, et al. Mol Ther. 2012, 20(4), 692-693. 2. Bronsart LL, et al. Mol Imaging Biol. 2015

Citations

1. Hong Y, Guo H, Wei M, et al. Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein. Journal of Virological Methods. 2022: 114564

Related compound libraries

This product is contained In the following compound libraries：

Marine Natural Product Library Natural Product Library for HTS Preclinical Compound Library NO PAINS Compound Library Bioactive Compounds Library Max Polyphenolic Natural Product Library RO5 Drug-like Natural Product Library Bioactive Compound Library Anti-Cardiovascular Disease Compound Library Human Metabolite Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Coelenterazine 55779-48-1 Others inhibit Inhibitor Oplophorus luciferin Coelenteramine inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.