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CYM-5541

Catalog No. T3961 CAS 945128-26-7

Synonyms: ML249, CYM 5541, CID-17253208

CYM-5541 is a selective and allosteric S1P3 receptor agonist.

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CYM-5541, CAS 945128-26-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 30.00
2 mg	In stock	$ 39.00
5 mg	In stock	$ 63.00
10 mg	In stock	$ 90.00
25 mg	In stock	$ 189.00
50 mg	In stock	$ 351.00
100 mg	In stock	$ 522.00
500 mg	In stock	$ 1,170.00
1 mL * 10 mM (in DMSO)	In stock	$ 68.00

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Purity: 98.39%

Purity: 97.34%

Biological Description

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Description	CYM-5541 is a selective and allosteric S1P3 receptor agonist.
Targets&IC50	S1P3:72-132 nM(EC50)
In vitro	CYM-5541 is a full agonist, able to reach the maximum level of ERK phosphorylation that is observed with S1P. CYM-5541 has an EC50 of between 72 and 132 nM and exhibits exquisite selectivity over other S1P receptor subtypes: S1P1 EC50>10 μM, S1P2 EC50>50 μM, S1P4 EC50>50 μM, and S1P5 EC50>25 μM. CYM-5541 also shows selectivity over a large panel of protein targets, with no significant activities, in the Ricerca profiling panel of 55 GPCRs, ion channels, and transporters. CYM-5541 allowed us to identify an allosteric site where F263 is a key gate-keeper residue for its affinity and efficacy. The novel allosteric hydrophobic pocket may account for the S1P3 selectivity of CYM-5541[1].
Kinase Assay	Jump-In TI CHO-K cells stably expressing WT or mutant S1P3 are serum-starved for 4 hrs. They are then incubated at 4 °C for 30 min in the binding buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 15 mM NaF, 0.5 mM EDTA, 1 mM Na3VO4, 0.5% fatty acid-free bovine serum albumin, and protease inhibitor mixture with 0.1 nM [33P]S1P and increasing concentrations of S1P, SPM-242, or CYM-5541. Cells are washed three times with cold binding buffer. Cell-bound radioactivity is measured by lysing the cells with 0.5% SDS followed by liquid scintillation counting. The raw data is normalized so that the level of [33P]S1P bound to each cell line (WT or mutant) in the absence of competing ligand is referenced as 100% for its own cell line[1].

Synonyms	ML249, CYM 5541, CID-17253208
Molecular Weight	316.44
Formula	C19H28N2O2
CAS No.	945128-26-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 2 years

Solubility Information

DMSO: 39 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Jo E, et al. Novel selective allosteric and bitopic ligands for the S1P(3) receptor. ACS Chem Biol. 2012 Dec 21;7(12):1975-83.

Related compound libraries

This product is contained In the following compound libraries：

NO PAINS Compound Library Target-Focused Phenotypic Screening Library Bioactive Compound Library Bioactive Compounds Library Max Anti-Obesity Compound Library GPCR Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

CYM-5541 945128-26-7 GPCR/G Protein S1P Receptor LPL Receptor CYM5541 inhibit ML-249 ML249 CYM 5541 ML 249 CID-17253208 Inhibitor Lysophospholipid Receptor inhibitor

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