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BAPTA-AM

Catalog No. T6245 CAS 126150-97-8

Synonyms: BAPTA/AM

BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling.

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BAPTA-AM, CAS 126150-97-8

Pack Size	Availability	Price/USD
5 mg	In stock	$ 30.00
10 mg	In stock	$ 51.00
25 mg	In stock	Inquiry
50 mg	In stock	Inquiry
1 mL * 10 mM (in DMSO)	In stock	$ 43.00

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Purity: 99.47%

Purity: 99.03%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling.
Targets&IC50	Kv1.3 (human, HEK293 cells):1.45 μM (IC50), Kv1.5 (human, HEK293 cells):1.23 μM (IC50), ERG channel (human, HEK293 cells):1.3 μM (IC50)
In vitro	METHODS: Chondrocytes were treated with BAPTA-AM (10 μM) and FAC (100 μM) for 24 h. Intracellular ROS levels were measured using the Reactive Oxygen Species Assay kit. RESULTS: FAC promoted ROS production and this effect was inhibited by the calcium chelator BAPTA-AM. [1] METHODS: Rat fibroblast RAT2 and Xenopus cells were treated with BAPTA-AM (50 μM) for 1 h, and microtubule depolymerization was detected by Immunostaining. RESULTS: BAPTA AM treatment for 30 min resulted in almost complete disassembly in most cells, and microtubules were uniformly depolymerized in cells within 60 min. [2]
In vivo	METHODS: To investigate the effect on ethanol-induced locomotor activity, BAPTA-AM (0-10 mg/kg, Cremophor EL 1.25% (v/v) in distilled water) was injected intraperitoneally into Swiss (RjOrl) mice, followed by ethanol (0-4 g/kg) 30 min later. RESULTS: Pretreatment with BAPTA-AM blocked the locomotor stimulus produced by ethanol without altering basal locomotion. On the contrary, BAPTA-AM reversed the ethanol-induced hypnosis. [3] METHODS: To investigate the effect on LPS-induced blood-brain barrier leakage, BAPTA-AM (12 mg/kg, 0.01% pluronic acid in sterile saline) was injected intravenously into FVB mice, followed 30 min later by intraperitoneal injection of LPS (25 mg/kg). RESULTS: BAPTA-AM reduced LPS-induced blood-brain barrier leakage. [4]

Synonyms	BAPTA/AM
Molecular Weight	764.68
Formula	C34H40N2O18
CAS No.	126150-97-8

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 145 mg/mL (189.62 mM）, Sonication is recommended.

References and Literature

1. Jing X, et al. Calcium chelator BAPTA‑AM protects against iron overload‑induced chondrocyte mitochondrial dysfunction and cartilage degeneration. Int J Mol Med. 2021 Oct;48(4):196. 2. Saoudi Y, et al. Calcium-independent cytoskeleton disassembly induced by BAPTA. Eur J Biochem. 2004 Aug;271(15):3255-64. 3. Baliño P, et al. Intracellular calcium chelation with BAPTA-AM modulates ethanol-induced behavioral effects in mice. Exp Neurol. 2012 Apr;234(2):446-53. 4. De Bock M, et al. Targeting gliovascular connexins prevents inflammatory blood-brain barrier leakage and astrogliosis. JCI Insight. 2022 Aug 22;7(16):e135263. 5. Tian C, Huang R, Tang F, et al. Transient Receptor Potential Ankyrin 1 Contributes to Lysophosphatidylcholine-Induced Intracellular Calcium Regulation and THP-1-Derived Macrophage Activation[J]. The Journal of Membrane Biology. 2019: 1-13. 6. Pan X, Li R, Guo H, et al. Dihydropyridine Calcium Channel Blockers Suppress the Transcription of PD-L1 by Inhibiting the Activation of STAT1[J]. Frontiers in Pharmacology. 2021, 11: 2233. 7. Yan T, Zhao Y. Acetaldehyde induces phosphorylation of dynamin-related protein 1 and mitochondrial dysfunction via elevating intracellular ROS and Ca2+ levels. Redox Biology. 2019: 101381. 8. Ge C, Huang H, Huang F, et al. Neurokinin-1 receptor is an effective target for treating leukemia by inducing oxidative stress through mitochondrial calcium overload[J]. Proceedings of the National Academy of Sciences. 2019: 201908998.

Citations

1. He C L, Huang L Y, Wang K, et al. Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products in vitro. Signal transduction and targeted therapy. 2021 Mar 23;6(1):131. 2. Yang Y, Yang P, Huang C, et al. Inhibitory effect on SARS-CoV-2 infection of neferine by blocking Ca2+ -dependent membrane fusion. Journal of Medical Virology. 2021, 93(10): 5825-5832 3. Ge C, Huang H, Huang F, et al. Neurokinin-1 receptor is an effective target for treating leukemia by inducing oxidative stress through mitochondrial calcium overload. Proceedings of the National Academy of Sciences. 2019, 116(39): 19635-19645 4. Yan T, Zhao Y. Acetaldehyde induces phosphorylation of dynamin-related protein 1 and mitochondrial dysfunction via elevating intracellular ROS and Ca2+ levels. Redox Biology. 2019: 101381 5. Zheng Q, Zou Y, Teng P, et al. Mechanosensitive Channel PIEZO1 Senses Shear Force to Induce KLF2/4 Expression via CaMKII/MEKK3/ERK5 Axis in Endothelial Cells. Cells. 2022, 11(14): 2191 6. Yi Y, Gao K, Zhang L, et al. Zearalenone Induces MLKL-Dependent Necroptosis in Goat Endometrial Stromal Cells via the Calcium Overload/ROS Pathway. International Journal of Molecular Sciences. 2022, 23(17): 10170. 7. Pan X, Li R, Guo H, et al. Dihydropyridine Calcium Channel Blockers Suppress the Transcription of PD-L1 by Inhibiting the Activation of STAT1. Frontiers in Pharmacology. 2021 Jan 13;11:539261. doi: 10.3389/fphar.2020.539261. eCollection 2020. 8. Tian C, Huang R, Tang F, et al. Transient Receptor Potential Ankyrin 1 Contributes to Lysophosphatidylcholine-Induced Intracellular Calcium Regulation and THP-1-Derived Macrophage Activation. The Journal of Membrane Biology. 2019: 1-13 9. Xia Q, Zheng H, Li Y, et al.SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis.Autophagy.2023: 1-17.

Related compound libraries

This product is contained In the following compound libraries：

Potassium Channel Blocker Library Anti-Cancer Compound Library Bioactive Compounds Library Max Anti-COVID-19 Compound Library Ion Channel Inhibitor Library Human Metabolite Library Bioactive Compound Library NO PAINS Compound Library Ferroptosis Compound Library

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Keywords

BAPTA-AM 126150-97-8 Membrane transporter/Ion channel Potassium Channel inhibit BAPTA/AM Inhibitor KcsA BAPTA AM BAPTAAM inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.