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BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
5 mg | $30 | In Stock | |
10 mg | $51 | In Stock | |
25 mg | Preferential | In Stock | |
50 mg | Preferential | In Stock | |
1 mL x 10 mM (in DMSO) | $43 | In Stock |
Description | BAPTA-AM is a calcium chelator that is cell-permeable and selective, blocking hERG, hKv1.3, and hKv1.5 channels (IC50=1.3/1.45/1.23 μM). BAPTA-AM has a 105-fold higher affinity for Ca2+ than for Mg2+, and can be used for the role of calcium in cell signaling. |
Targets&IC50 | ERG channel (human, HEK293 cells):1.3 μM, Kv1.5 (human, HEK293 cells):1.23 μM, Kv1.3 (human, HEK293 cells):1.45 μM |
In vitro | METHODS: Chondrocytes were treated with BAPTA-AM (10 μM) and FAC (100 μM) for 24 h. Intracellular ROS levels were measured using the Reactive Oxygen Species Assay kit. RESULTS: FAC promoted ROS production and this effect was inhibited by the calcium chelator BAPTA-AM. [1] METHODS: Rat fibroblast RAT2 and Xenopus cells were treated with BAPTA-AM (50 μM) for 1 h, and microtubule depolymerization was detected by Immunostaining. RESULTS: BAPTA AM treatment for 30 min resulted in almost complete disassembly in most cells, and microtubules were uniformly depolymerized in cells within 60 min. [2] |
In vivo | METHODS: To investigate the effect on ethanol-induced locomotor activity, BAPTA-AM (0-10 mg/kg, Cremophor EL 1.25% (v/v) in distilled water) was injected intraperitoneally into Swiss (RjOrl) mice, followed by ethanol (0-4 g/kg) 30 min later. RESULTS: Pretreatment with BAPTA-AM blocked the locomotor stimulus produced by ethanol without altering basal locomotion. On the contrary, BAPTA-AM reversed the ethanol-induced hypnosis. [3] METHODS: To investigate the effect on LPS-induced blood-brain barrier leakage, BAPTA-AM (12 mg/kg, 0.01% pluronic acid in sterile saline) was injected intravenously into FVB mice, followed 30 min later by intraperitoneal injection of LPS (25 mg/kg). RESULTS: BAPTA-AM reduced LPS-induced blood-brain barrier leakage. [4] |
Cell Research | Instructions for use I. Solution preparation 1. Prepare BAPTA-AM storage solution: Dissolve BAPTA-AM in anhydrous DMSO to prepare a 50 mM storage solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting). 2. Prepare working solution: Dilute BAPTA-AM storage solution into cell culture medium to a final concentration of 6-50 μM. II. Cell preparation 1. Grow cells to an appropriate density. For adherent cells, they can be planted in culture dishes or culture plates; for suspended cells, adjust the cell concentration to 2×10^6 cells/mL. 2. Incubate cells with BAPTA-AM solution, usually at 37℃, 5% CO₂ for 30-60 minutes, with gentle shaking during the period. 3. Remove excess dye: After incubation, wash cells with calcium-free buffer to remove BAPTA-AM that has not entered the cells. 4. For adherent cells, they can be directly washed with calcium-free PBS; for suspended cells, the cells need to be collected by centrifugation and then resuspended with calcium-free PBS. 5. Detection of changes in calcium ion concentration: You can choose the appropriate detection method according to the experimental requirements: Fluorescence microscope detection: observe the changes in fluorescence intensity in cells. The decrease in fluorescence intensity indicates a decrease in calcium ion concentration. Flow cytometry detection: analyze the changes in fluorescence intensity in cells through flow cytometry. Fluorescence spectrometer detection: measure the changes in fluorescence intensity of cell suspension. |
Alias | BAPTA/AM |
Molecular Weight | 764.68 |
Formula | C34H40N2O18 |
Cas No. | 126150-97-8 |
Smiles | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc1ccccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O |
Relative Density. | 1.35 g/cm3 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | ||||||||||||||||||||||||||||||
Solubility Information | DMSO: 50 mg/mL (65.39 mM), Sonication is recommended. ![]() | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
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