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Amonafide

Catalog No. T6336 CAS 69408-81-7

Synonyms: NSC308847,AS1413, Nafidimide, AS1413, Quinamed

Amonafide (NSC-308847,AS1413)(AS1413) produces protein-associated DNA-strand breaks through a topoisomerase II-mediated reaction, but does not produce topoisomerase I-mediated DNA cleavage.

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Amonafide, CAS 69408-81-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 33.00
2 mg	In stock	$ 47.00
5 mg	In stock	$ 73.00
10 mg	In stock	$ 89.00
25 mg	In stock	$ 166.00
50 mg	In stock	$ 296.00
100 mg	In stock	$ 491.00
1 mL * 10 mM (in DMSO)	In stock	$ 84.00

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Purity: 98%

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Description	Amonafide (NSC-308847,AS1413)(AS1413) produces protein-associated DNA-strand breaks through a topoisomerase II-mediated reaction, but does not produce topoisomerase I-mediated DNA cleavage.
In vitro	Through a topoisomerase II-mediated reaction, Amonafide treatment produces DNA single-strand breaks (SSB), double-strand breaks (DSB), and DNA-protein cross-links in human myeloid leukemia cells. Amonafide treatment inhibits conlony formation of the leukemic cell lines and the normal human bone marrow GM-CFC in a dose-dependent manner. Amonafide does not produce topoisomerase I-mediated DNA cleavage even at 100 μM. The m-AMSA-resistant line is less than 2-fold resistant to Amonafide [1] Amonafide interferes with the DNA breakage-reunion activity of mammalian DNA topoisomerase II resulting in DNA cleavage stimulation. [2] Compared with those of other antitumor drugs, Amonafide-stimulated cleavage intensity patterns are markedly different. Amonafide highly prefers a cytosine, and excludes guanines and thymines instead, at position -1, with lower preference for an adenine at position +1. [3] Topoisomerase II-mediated DNA cleavage induced by Amonafide is affected only slightly (less than 3-fold) by 1 mM ATP, suggeting that Amonafide is an ATP-insensitive topoisomerase II inhibitor in contrast to doxorubicin, etoposide, and mitoxantrone. [4] Amonafide significantly inhibits the growth of HT-29, HeLa, and PC3 cells with IC50 of 4.67 μM, 2.73 μM, and 6.38 μM, respectively. [5] Amonafide is unaffected by P-glycoprotein-mediated efflux, unlike those of the classical topoisomerase II inhibitors (daunorubicin, doxorubicin, idarubicin, etoposide, and mitoxantrone). [6]
Cell Research	All cell lines are in the logarithmic phase of growth when the assay of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is carried out. Cells are harvested and seeded into 96-well tissue culture plates at a density of 2.5 × 103 cells/well in 150 μL aliquots of medium. The concentrations tested are serial dilutions of a stock solution (10 μM in DMSO) with phosphate-buffered saline (PBS) and are added 24 hours later. The assay is ended after 72 hours of Amonafide exposure and PBS is used as a negative control. After 72 hours treatment, cells are washed twice with PBS, and then 50 μL/well of MTT reagent (1 mg/mL in PBS) together with 150 μL/well of prewarmed medium are added. The plates are returned to the incubator for 4 hours. Subsequently, DMSO is added as solvent. Absorbance is determined at 570 nm with a Microplate reader. All experiments are performed at least three times, and the average of the percentage absorbance is plotted against concentration. Then, the concentration of Amonafide required to inhibit 50% of cell growth (IC50) is calculated for Amonafide.(Only for Reference)

Synonyms	NSC308847,AS1413, Nafidimide, AS1413, Quinamed
Molecular Weight	283.33
Formula	C16H17N3O2
CAS No.	69408-81-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 53 mg/mL (187.1 mM)

Ethanol: 4 mg/mL (14.11 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Andersson BS, et al. Cancer Res, 1987, 47(4), 1040-1044. 2. Hsiang YH, et al. Mol Pharmacol, 1989, 36(3), 371-376. 3. De Isabella P, Nucleic Acids Res, 1995, 23(2), 223-229. 4. Wang H, et al. J Biol Chem, 2001, 276(19), 151990-151995. 5. BrañaMF, et al. J Med Chem, 2004, 47(6), 1391-1399.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Anti-Cancer Drug Library Anti-Cancer Active Compound Library Anti-Cancer Clinical Compound Library Inhibitor Library ReFRAME Related Library Anti-Prostate Cancer Compound Library Bioactive Compound Library Anti-Breast Cancer Compound Library DNA Damage & Repair Compound Library

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Keywords

Amonafide 69408-81-7 DNA Damage/DNA Repair Topoisomerase NSC308847,AS-1413 AS 1413 inhibit NSC308847,AS1413 AS-1413 NSC308847,AS 1413 Nafidimide Inhibitor AS1413 Quinamed inhibitor

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