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9-Aminoacridine

Name: 9-Aminoacridine
Brand: TargetMol
SKU: T0289
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0289Cas No. 90-45-9

Alias Aminacrine

9-Aminoacridine is a highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.

9-Aminoacridine

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Purity: 99.93%

Catalog No. T0289Alias AminacrineCas No. 90-45-9

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$30	In Stock	In Stock
500 mg	$42	-	In Stock
1 g	$64	-	In Stock
2 g	$91	-	In Stock
5 g	$148	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.93%

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COA HNMR HPLC

Product Introduction

9-Aminoacridine AI Summary

9-Aminoacridine exhibits a diverse range of bioactivities with significant implications across various biological systems. It shows a high binding affinity to DNA, with a DNA dissociation constant (Log Kd) of 5.7 and a strong association constant (Log K) of 6.08. The compound displaces ethidium bromide from DNA, indicating its potential for DNA targeting applications. It demonstrates cytotoxic effects against various cell lines, including an IC50 of 2600.0 nM in L1210 leukemia cells, 2140.0 nM in human peripheral blood monocytes, and 2000.0 nM in human HL60 cells. In vivo, it has an optimal non-toxic dose of 20 mg/kg intraperitoneally and increases the life span of treated animals injected with P388 leukemia by 20%. 9-Aminoacridine also shows bioactivity in enzyme inhibition; for instance, it is an inhibitor of hydroxysteroid dehydrogenase 4 (HSD17B4), human Jumonji domain-containing 2E (JMJD2E), caspase-1, caspase-7, tyrosyl-DNA phosphodiesterase (TDP1), aldose reductase, and MAO enzymes, with particularly notable selectivity towards MAO-B over MAO-A. It inhibits the apamin-sensitive SKCa channel in guinea-pig hepatocytes with an IC50 of 120,000 nM and OATP1B3 with 80.82% inhibition at 10 µM. Additionally, the compound exhibits antiviral activity, including moderate to high inhibition rates against SARS-CoV-2, and shows genotoxicity in Salmonella typhimurium. It demonstrates a broad spectrum of enzyme inhibition and receptor binding, affecting human DRD1, ADRA2A, OPRM1, and other receptors. Its lipophilicity value (Rm) is -0.08, and it undergoes ionization with a pKa of 9.6. These multifaceted bioactivities suggest that 9-Aminoacridine has potential applications in cancer therapy, antiviral treatments, enzyme inhibition strategies, and possibly as an agent for DNA targeting and manipulation..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	9-Aminoacridine is a highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.
Cell Research	I. Solution preparation 1. Preparation of stock solution: Dissolve it in DMSO (dimethylsulfonamide) or water to prepare the stock solution, usually at a concentration of 1-10mM. It can be adjusted according to actual conditions. 2. Preparation of working solution: Dilute it to an appropriate concentration with PBS/DMEM/H2O before use. The common working concentration is 1-10 μM, but the specific concentration should be optimized according to experimental requirements. II. Cell labeling 1) Cell culture: Inoculate cells into appropriate culture dishes and culture to an appropriate density (e.g. 24 hours, the number of cells is 70%-80% density). 2) Labeling cells: Add a working concentration of 9-Aminoacridine solution to the cell culture medium. The general incubation time is 30 minutes to 1 hour, which can be adjusted according to experimental requirements. 3) Washing cells: After labeling, wash the cells thoroughly with PBS (phosphate buffered saline) to remove unbound 9-Aminoacridine. 4) Fluorescence detection: Excitation and emission wavelengths: 9-Aminoacridine is excited at ex = 365 nm and emits fluorescence at em = 460 nm, producing bright blue fluorescence. Fluorescence microscopy or flow cytometry: Use a suitable fluorescence microscope or flow cytometer for fluorescence detection to observe the fluorescence intensity in the cells. Precautions: 1. Protect from light: 9-Aminoacridine is sensitive to light, especially when exposed to strong light, the fluorescence intensity will change. Avoid exposure to strong light when using it. 2. Solubility: It has different solubility in different solvents, and the solubility needs to be confirmed before use. 3. Cytotoxicity: Higher concentrations of 9-Aminoacridine may be toxic to cells, so the concentration should be optimized to avoid excessive cell toxicity. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Aminacrine

Chemical Properties

Molecular Weight	194.23
Formula	C₁₃H₁₀N₂
Cas No.	90-45-9
Smiles	NC1=C2C=CC=CC2=NC2=C1C=CC=C2
Relative Density.	1.268g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (257.43 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (12.87 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.1485 mL	25.7427 mL	51.4854 mL	257.4268 mL
5 mM	1.0297 mL	5.1485 mL	10.2971 mL	51.4854 mL
10 mM	0.5149 mL	2.5743 mL	5.1485 mL	25.7427 mL
20 mM	0.2574 mL	1.2871 mL	2.5743 mL	12.8713 mL
50 mM	0.1030 mL	0.5149 mL	1.0297 mL	5.1485 mL
100 mM	0.0515 mL	0.2574 mL	0.5149 mL	2.5743 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc