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Chromatin/Epigenetic Sirtuin 3-TYP

3-TYP

Catalog No. T4108   CAS 120241-79-4
Synonyms: 3-(1H-1,2,3-triazol-4-yl) pyridine
Purity 100.00% Datasheet

3-TYP is a selective SIRT3 inhibitor.

3-TYP, CAS 120241-79-4
Pack Size Availability Price/USD Quantity
5 mg In stock 58.00
10 mg In stock 99.00
25 mg In stock 198.00
50 mg In stock 306.00
100 mg In stock 550.00
1 mL * 10 mM (in DMSO) In stock 58.00
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Purity 100.00%
Biological Description
Chemical Properties
Storage & Solubility Information
Description 3-TYP is a selective SIRT3 inhibitor.
Targets&IC50 SIRT1 :ic50 88nM,   SIRT2 :ic50 92nM,   SIRT3 :ic50 16nM,  
In vivo 3-TYP significantly decreases SIRT3 activity and increases the acetylation of SOD2 compared with that in the control group, without influencing SIRT3 expression. 3-TYP attenuates the cardioprotective effects of melatonin by decreasing the LVEF and LVFS after 24 hours of reperfusion. 3-TYP also increases the infarct size, serum LDH levels, and apoptotic ratio compared with those in the IR+Mel group. 3-TYP (50 mg/kg, i.p.) does not significantly influence the LVEF, LVFS, infarct size, serum LDH levels, apoptosis, and oxidative stress compared with those of the Sham group. Moreover, 3-TYP has little effect on gp91phox, Nrf2, NQO 1, Bax, Bcl-2, Caspase-3, and cleaved Caspase-3 expression levels, compared with the Sham group.
Animal Research
3-TYP is formulated in 1% ethanol. Male C57BL/6 mice are anesthetized with 2% isoflurane, and myocardial ischemia is produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 minutes of myocardial ischemia, the slipknot is released, and the myocardium is reperfused for 3 hour (for western blot analysis and oxidative stress measurement) or 24 hour (for cardiac function, apoptotic index and infarct size determination). Sham-operated mice undergo the same surgical procedures except the suture placed under the left coronary artery is not tied. Ten minutes before reperfusion, mice are randomized to receive either vehicle (1% ethanol) or melatonin (20 mg/kg) by intraperitoneal injection. C57BL/6 mice are randomly divided into the following groups: (i) Sham group: mice underwent the sham operation and are treated with vehicle (1% ethanol); (ii) Mel group: mice are treated with melatonin (20 mg/kg via intraperitoneal injection); (iii) IR+V group: mice underwent the MI/R operation and are treated with vehicle (1% ethanol); (iv) IR+Mel group: mice underwent the MI/R operation and are treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); (v) IR+Mel+3-TYP group: mice are pretreated with 3-TYP (3-TYP is intraperitoneally injected at a dose of 50 mg/kg every 2 days for a total of three doses prior to the MI/R surgery), subjected to the MI/R operation, and treated with melatonin (20 mg/kg via intraperitoneal injection 10 minutes before reperfusion); and (vi) IR+3-TYP group: mice are pretreated with 3-TYP and then subjected to the MI/R operation.
Cell Research
Cell viability is analyzed using Cell Counting Kit-8. 1×104 cells are inoculated into 96-well plates. After being treated, 90 μL of medium and 10 μL of CCK-8 solution are added to each well. The cells are then incubated at 37°C for 2 h. After incubation, the absorption at 450 nm is measured using an Infinite™ M200 Microplate Reader. The results are expressed as a percentage of the control. The cell death is also evaluated using the trypan blue assay. HepG2 cells are plated in the 6-well plates (5×105 cells per well) and incubated for 24 h. After being treated with Cd or melatonin, the cells are detached with 300 μL trypsin-EDTA solution. The mixture of detached cells is centrifugated at 300 g for 5 min. Then, the residue is combined with 800 μL trypan blue solution and dispersed. After 3 min staining, cells are counted using an automated cell counter. The dead cells are stained with the blue color. Cell mortality (%) is expressed as percentage of the dead cell number/the total cell number.
Synonyms 3-(1H-1,2,3-triazol-4-yl) pyridine
Purity 100.00%
Appearance solid
Molecular Weight 146.15
Formula C7H6N4
CAS No. 120241-79-4

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 28 mg/mL (191.6 mM)

Ethanol: 28 mg/mL (191.6 mM)

Water: 5 mg/mL (34.21 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Pi H, et al. SIRT3-SOD2-mROS-dependent autophagy in cadmium-induced hepatotoxicity and salvage by melatonin. Autophagy. 2015;11(7):1037-51 2. Zhai M, et al. Melatonin ameliorates myocardial ischemia reperfusion injury through SIRT3-dependent regulation of oxidative stress and apoptosis. J Pineal Res. 2017 Sep;63(2).

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Anti-cancer Compound Library Epigenetics Compound Library DNA Damage & Repair Compound Library Histone Modification Research Compound Library

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