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Withanolide B

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Catalog No. TN2310Cas No. 56973-41-2

Withanolide B, a natural product isolated from Withania somnifera Dunal, promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) via the ERK1/2 and Wnt/β-catenin signalling pathways, thereby enhancing bone healing. Withanolide B exhibits affinity for the catalytic domain of PARP-1.

Withanolide B

Withanolide B

😃Good
Catalog No. TN2310Cas No. 56973-41-2
Withanolide B, a natural product isolated from Withania somnifera Dunal, promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) via the ERK1/2 and Wnt/β-catenin signalling pathways, thereby enhancing bone healing. Withanolide B exhibits affinity for the catalytic domain of PARP-1.
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1 mg$3326-8 weeks
5 mg$9326-8 weeks
10 mgInquiry6-8 weeks
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Product Introduction

Bioactivity
Description
Withanolide B, a natural product isolated from Withania somnifera Dunal, promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) via the ERK1/2 and Wnt/β-catenin signalling pathways, thereby enhancing bone healing. Withanolide B exhibits affinity for the catalytic domain of PARP-1.
Targets&IC50
SK-N-SH cells:14.84±1.45 μM
In vitro
Methods: SK-N-SH cells were treated with Withanolide B (10-100 μM, 48 hours), and cell viability was assessed using the MTT assay.
Results: The IC50 value of Withanolide B for SK-N-SH cells was 14.84±1.45 μM, indicating that Withanolide B exhibits low toxicity to neuronal cells. [1]
In vivo
Methods: By injecting Withanolide B (10 mg/kg) into the bone defect site of the SD rat tibial osteotomy model, the effect of Withanolide B on the osteogenic differentiation of hBMSCs was investigated. Results: Micro-CT scanning and histological analysis demonstrated that Withanolide B significantly promoted the healing of bone defects. [2]
Chemical Properties
Molecular Weight454.6
FormulaC28H38O5
Cas No.56973-41-2
Smiles[H][C@@]1(CC[C@@]2([H])[C@]3([H])[C@@H]4O[C@@H]4[C@@]4(O)CC=CC(=O)[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)[C@@]1([H])CC(C)=C(C)C(=O)O1
Relative Density.1.31g/cm3
ColorWhite
AppearanceSolid
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
Acetone: 0.8 mg/mL (1.76 mM), Sonication is recommended.
DMSO: 2.5 mg/mL (5.5 mM), Sonication is recommended.
Solution Preparation Table
Acetone/DMSO
1mg5mg10mg50mg
1 mM2.1997 mL10.9987 mL21.9974 mL109.9868 mL
DMSO
1mg5mg10mg50mg
5 mM0.4399 mL2.1997 mL4.3995 mL21.9974 mL

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Withanolide B
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