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SR12418

Name: SR12418
Brand: TargetMol
SKU: T63206
Price: 71 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T63206Cas No. 1801185-08-9

Alias SR-12418, SR 12418

SR12418 is a specific synthetic ligand for REV-ERB, demonstrating binding affinity for both REV-ERBα (IC50 = 68 nM) and REV-ERBβ (IC50 = 119 nM) in TR-FRET assays. SR12418 inhibits IL-17A expression in EL4 cells and suppresses TH17 cell differentiation, making it suitable for studies on experimental autoimmune encephalomyelitis (EAE) and colitis.

SR12418

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Purity: 99.96%

Catalog No. T63206Alias SR-12418, SR 12418Cas No. 1801185-08-9

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$71	-	In Stock
2 mg	$98	-	In Stock
5 mg	$172	-	In Stock
10 mg	$289	-	In Stock
25 mg	$579	-	In Stock
1 mL x 10 mM (in DMSO)	$183	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.96%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	SR12418 is a specific synthetic ligand for REV-ERB, demonstrating binding affinity for both REV-ERBα (IC50 = 68 nM) and REV-ERBβ (IC50 = 119 nM) in TR-FRET assays. SR12418 inhibits IL-17A expression in EL4 cells and suppresses TH17 cell differentiation, making it suitable for studies on experimental autoimmune encephalomyelitis (EAE) and colitis.
Targets&IC50	REV-ERBα:68 nM, REV-ERBβ:119 nM
In vitro	SR12418 at a concentration of 5 μM for 96 hours can inhibit the growth of TH17 cells [1]. Under TH17 polarization induction conditions, SR12418 (5 and 10 μM, 4 days) can suppress cell differentiation [1].
In vivo	SR12418 (50 mg/kg, intraperitoneal injection, twice daily) can inhibit the onset and disease severity of experimental autoimmune encephalomyelitis (EAE) [1]. Starting from day 18 post-immunization, SR12418 (50 mg/kg, intraperitoneal injection, twice daily) demonstrates therapeutic effects in the intervention study of relapsing-remitting experimental autoimmune encephalomyelitis [1].
Synonyms	SR-12418, SR 12418

Chemical Properties

Molecular Weight	483.57
Formula	C₃₁H₃₀FNO₃
Cas No.	1801185-08-9
Smiles	C(=O)(N1[C@H](COC2=CC=C(OC(C)(C)C)C=C2)CC=3C(C1)=CC=C(F)C3)C=4C5=C(C=CC4)C=CC=C5

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (165.44 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (6.82 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0680 mL	10.3398 mL	20.6795 mL	103.3976 mL
5 mM	0.4136 mL	2.0680 mL	4.1359 mL	20.6795 mL
10 mM	0.2068 mL	1.0340 mL	2.0680 mL	10.3398 mL
20 mM	0.1034 mL	0.5170 mL	1.0340 mL	5.1699 mL
50 mM	0.0414 mL	0.2068 mL	0.4136 mL	2.0680 mL
100 mM	0.0207 mL	0.1034 mL	0.2068 mL	1.0340 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

SR12418

SR12418

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