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SMER28

Name: SMER28
Brand: TargetMol
SKU: T3155
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3155Cas No. 307538-42-7

SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells via an mTOR-independent mechanism and prevented the accumulation of amyloid beta peptide.

SMER28

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Purity: 99.94%

Catalog No. T3155Cas No. 307538-42-7

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$45	In Stock	In Stock
10 mg	$67	In Stock	In Stock
25 mg	$129	In Stock	In Stock
50 mg	$243	In Stock	In Stock
100 mg	$392	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$47	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.94%

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells via an mTOR-independent mechanism and prevented the accumulation of amyloid beta peptide.
In vitro	SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment[1].
Cell Research	MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.(Only for Reference)

Chemical Properties

Molecular Weight	264.12
Formula	C₁₁H₁₀BrN₃
Cas No.	307538-42-7
Smiles	BrC1=CC=C2N=CN=C(NCC=C)C2=C1
Relative Density.	1.520 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (227.17 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.57 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7862 mL	18.9308 mL	37.8616 mL	189.3079 mL
5 mM	0.7572 mL	3.7862 mL	7.5723 mL	37.8616 mL
10 mM	0.3786 mL	1.8931 mL	3.7862 mL	18.9308 mL
20 mM	0.1893 mL	0.9465 mL	1.8931 mL	9.4654 mL
50 mM	0.0757 mL	0.3786 mL	0.7572 mL	3.7862 mL
100 mM	0.0379 mL	0.1893 mL	0.3786 mL	1.8931 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc