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Sabutoclax

Name: Sabutoclax
Brand: TargetMol
SKU: T6650
Price: 64 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6650Cas No. 1228108-65-3

Alias BI-97C1

Sabutoclax (BI-97C1)(BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.

Sabutoclax

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Purity: 99.3%

Catalog No. T6650Alias BI-97C1Cas No. 1228108-65-3

Sabutoclax (BI-97C1)(BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$64	-	In Stock
5 mg	$155	-	In Stock
10 mg	$247	-	In Stock
25 mg	$497	-	In Stock
50 mg	$788	-	In Stock
100 mg	$1,070	-	In Stock
1 mL x 10 mM (in DMSO)	$169	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

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Purity:99.3%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS ORP

Product Introduction

Bioactivity

Description	Sabutoclax (BI-97C1)(BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.
Targets&IC50	Bfl-1:0.62 μM, MCL1:0.2 μM, BCL-XL:0.31 μM, Bcl-2:0.32 μM
In vitro	Sabutoclax (0.001-10 μM; 72 hours) inhibits the growth of human prostate cancer and lung cancer cell lines[2]. Sabutoclax (0.01-1 μM; 24-48 hours) induces apoptosis in human diffuse large B-cell lymphoma cells[2]. Sabutoclax (0-15 μM; 48 hours) upregulates the levels of pro-apoptotic proteins in chemotherapy-resistant cells[1].
In vivo	Sabutoclax (1-5 mg/kg; intraperitoneal injection; every two days for a total of 18 days) reduces the growth of M2182 tumors in nude mice[2].
Kinase Assay	Competitive fluorescence polarization assays (FPA) : A Bak BH3 peptide (F-BakBH3) (GQVGRQLAIIGDDINR) is labeled at the N-terminus with fluorescein isothiocyanate (FITC) and purified by HPLC. For competitive binding assays, 100 nM GST-Bcl-XL ΔTM protein is preincubated with the tested compound at varying concentrations in 47.5 μL PBS (pH = 7.4) in 96-well black plates at room temperature for 10 min, and then 2.5 μL of 100 nM FITC-labeled Bak BH3 peptide is added to produce a final volume of 50 μL. The wild-type and mutant Bak BH3 peptides are included in each assay plate as positive and negative controls, respectively. After 30 min incubation at room temperature, the polarization values in millipolarization units are measured at excitation/emission wavelengths of 480/535 nm with a multilabel plate reader. IC50 is determined by fitting the experimental data to a sigmoidal dose-response nonlinear regression model. Data reported are mean of three independent experiments. Performance of Bcl-2 and Mcl-1FPA are similar. Briefly, 50 nM of GST-Bcl-2 or -Mcl-1are incubatedwith various concentrations of compound (4 and 11-14) for 2 min, and then 15 nM FITC-conjugated-Bim BH3 peptide is added in PBS buffer. Fluorescence polarization is measured after 10 min.
Cell Research	ATP-LITE assay(Only for Reference)
Synonyms	BI-97C1

Chemical Properties

Molecular Weight	700.78
Formula	C₄₂H₄₀N₂O₈
Cas No.	1228108-65-3
Smiles	O=C(C1=C2C=C(C)[C@]([C@]3=C(C)C=C4C(C(NC[C@H](C)C5=CC=CC=C5)=O)=C(O)C(O)=CC4=C3O)=C(O)C2=CC(O)=C1O)NC[C@H](C)C6=CC=CC=C6
Relative Density.	1.358 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 22 mg/mL (31.39 mM), Sonication is recommended.

DMSO: 93 mg/mL (132.71 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 3.3 mg/mL (4.71 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4270 mL	7.1349 mL	14.2698 mL	71.3491 mL
5 mM	0.2854 mL	1.4270 mL	2.8540 mL	14.2698 mL
10 mM	0.1427 mL	0.7135 mL	1.4270 mL	7.1349 mL
20 mM	0.0713 mL	0.3567 mL	0.7135 mL	3.5675 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0285 mL	0.1427 mL	0.2854 mL	1.4270 mL
100 mM	0.0143 mL	0.0713 mL	0.1427 mL	0.7135 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc