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NHI-2

Name: NHI-2
Brand: TargetMol
SKU: T24530
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T24530Cas No. 1269802-97-2

Alias NHI2

NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 value of 14.7 µM, demonstrating high selectivity for LDHA over LDHB, for which the IC50 is 55.8 µM. NHI-2 functions as an efficient anti-glycolytic agent, enhancing apoptosis, inducing cell cycle arrest at the S and G2 phases, and exerting broad-spectrum antiproliferative effects in cancer cells. NHI-2 additionally affects extracellular acidification rates and ATP production and has demonstrated tumor growth suppression in murine B78 melanoma models, highlighting its role in cancer metabolism research.

NHI-2

🥰Excellent

Purity: 99.981%

Catalog No. T24530Alias NHI2Cas No. 1269802-97-2

Pack Size	Price	Availability
1 mg	$39	In Stock
5 mg	$89	In Stock
10 mg	$139	In Stock
25 mg	$278	In Stock
50 mg	$417	In Stock
100 mg	$593	In Stock
200 mg	$859	In Stock

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Purity:99.981%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 value of 14.7 µM, demonstrating high selectivity for LDHA over LDHB, for which the IC50 is 55.8 µM. NHI-2 functions as an efficient anti-glycolytic agent, enhancing apoptosis, inducing cell cycle arrest at the S and G2 phases, and exerting broad-spectrum antiproliferative effects in cancer cells. NHI-2 additionally affects extracellular acidification rates and ATP production and has demonstrated tumor growth suppression in murine B78 melanoma models, highlighting its role in cancer metabolism research.
Targets&IC50	LDHA:14.7 µM
In vitro	Methods: B78 cells were treated with NHI-2 (12-40 μM, 72 h), and cell proliferation was monitored in real-time using the IncuCyte™ S3 live-cell imaging system. Results: NHI-2 completely inhibited cell proliferation at 40 μM, with an EC50 value of 25 μM. [6]
In vivo	Methods: NHI-2 (1 mg/kg, once daily for 15 consecutive days) was administered via intratumoral injection to B78 melanoma mouse models to evaluate the efficacy of lactic acid dehydrogenase inhibitors (LDHIs) in combination with immune checkpoint inhibitors (ICIs) and radiotherapy (RT). Results: The combined use of LDHIs, ICIs, and RT significantly reduced tumor volume and increased the CD8:Treg cell ratio, indicating enhanced anti-tumor immune response. [6]
Synonyms	NHI2

Chemical Properties

Molecular Weight	335.28
Formula	C₁₇H₁₂F₃NO₃
Cas No.	1269802-97-2
Smiles	C(F)(F)(F)C1=C2C(N(O)C(C(OC)=O)=C2)=CC(=C1)C3=CC=CC=C3
Relative Density.	1.36 g/cm3 (Predicted)
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (59.65 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9826 mL	14.9129 mL	29.8258 mL	149.1291 mL
5 mM	0.5965 mL	2.9826 mL	5.9652 mL	29.8258 mL
10 mM	0.2983 mL	1.4913 mL	2.9826 mL	14.9129 mL
20 mM	0.1491 mL	0.7456 mL	1.4913 mL	7.4565 mL
50 mM	0.0597 mL	0.2983 mL	0.5965 mL	2.9826 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

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DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

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