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NHI-2

Name: NHI-2
Brand: TargetMol
SKU: T24530
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: NHI2) Copy Product Info

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Synonyms: NHI2

Catalog No. T24530 Copy Product Info

Purity: 99.98%

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NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 value of 14.7 µM, demonstrating high selectivity for LDHA over LDHB, for which the IC50 is 55.8 µM. NHI-2 functions as an efficient anti-glycolytic agent, enhancing apoptosis, inducing cell cycle arrest at the S and G2 phases, and exerting broad-spectrum antiproliferative effects in cancer cells. NHI-2 additionally affects extracellular acidification rates and ATP production and has demonstrated tumor growth suppression in murine B78 melanoma models, highlighting its role in cancer metabolism research.

Cas No. 1269802-97-2

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Pack Size	Price	USA Stock	Global Stock
1 mg	$39	-	In Stock
5 mg	$89	-	In Stock
10 mg	$139	-	In Stock
25 mg	$278	-	In Stock
50 mg	$417	-	In Stock
100 mg	$593	-	In Stock
200 mg	$859	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.98%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 value of 14.7 µM, demonstrating high selectivity for LDHA over LDHB, for which the IC50 is 55.8 µM. NHI-2 functions as an efficient anti-glycolytic agent, enhancing apoptosis, inducing cell cycle arrest at the S and G2 phases, and exerting broad-spectrum antiproliferative effects in cancer cells. NHI-2 additionally affects extracellular acidification rates and ATP production and has demonstrated tumor growth suppression in murine B78 melanoma models, highlighting its role in cancer metabolism research.
Targets & IC50	LDHA:14.7 µM
In vitro	Methods: B78 cells were treated with NHI-2 (12-40 μM, 72 h), and cell proliferation was monitored in real-time using the IncuCyte™ S3 live-cell imaging system. Results: NHI-2 completely inhibited cell proliferation at 40 μM, with an EC50 value of 25 μM. [6]
In vivo	Methods: NHI-2 (1 mg/kg, once daily for 15 consecutive days) was administered via intratumoral injection to B78 melanoma mouse models to evaluate the efficacy of lactic acid dehydrogenase inhibitors (LDHIs) in combination with immune checkpoint inhibitors (ICIs) and radiotherapy (RT). Results: The combined use of LDHIs, ICIs, and RT significantly reduced tumor volume and increased the CD8:Treg cell ratio, indicating enhanced anti-tumor immune response. [6]
Synonyms	NHI2

Chemical Properties

Molecular Weight	335.28
Formula	C₁₇H₁₂F₃NO₃
Cas No.	1269802-97-2
Smiles	C(F)(F)(F)C1=C2C(N(O)C(C(OC)=O)=C2)=CC(=C1)C3=CC=CC=C3
Relative Density.	1.36 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (59.65 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9826 mL	14.9129 mL	29.8258 mL	149.1291 mL
5 mM	0.5965 mL	2.9826 mL	5.9652 mL	29.8258 mL
10 mM	0.2983 mL	1.4913 mL	2.9826 mL	14.9129 mL
20 mM	0.1491 mL	0.7456 mL	1.4913 mL	7.4565 mL
50 mM	0.0597 mL	0.2983 mL	0.5965 mL	2.9826 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc