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KX1-004

Name: KX1-004
Brand: TargetMol
SKU: T1812
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1812Cas No. 518058-84-9

Alias KX1004, KX1 004

KX1-004, a potential protective drug for NIHL, is an effective small molecule inhibitor of Src-PTK.

KX1-004

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Purity: 99.84%

Catalog No. T1812Alias KX1004, KX1 004Cas No. 518058-84-9

KX1-004, a potential protective drug for NIHL, is an effective small molecule inhibitor of Src-PTK.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$38	In Stock	In Stock
2 mg	$54	In Stock	In Stock
5 mg	$89	In Stock	In Stock
10 mg	$163	In Stock	In Stock
25 mg	$297	In Stock	In Stock
50 mg	$490	In Stock	In Stock
100 mg	$690	In Stock	In Stock
500 mg	$1,450	-	In Stock
1 mL x 10 mM (in DMSO)	$98	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.84%

Appearance:Solid

Color:White to Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	KX1-004, a potential protective drug for NIHL, is an effective small molecule inhibitor of Src-PTK.
Targets&IC50	Src PTK:40 μM
Kinase Assay	The Lck HTRF kinase assay involves ATP-dependent phosphorylation of a biotinylated substrate peptide of gastrin in the presence or absence of inhibitor compound. The final concentration of gastrin is 1.2 μM. The final concentration of ATP is 0.5 μM (Km app =0.6±0.1 μM), and the final concentration of Lck (a GST-kinase domain fusion (AA 225?509)) is 250 pM. Buffer conditions are as follows: 50 mM HEPES pH=7.5, 50 mM NaCl, 20 mM MgCl2, 5 mM MnCl2, 2 mM DTT, 0.05% BSA. The assay is quenched and stopped with 160 μL of detection reagent. Detection reagents are as follows: Buffer made of 50 mM Tris, pH=7.5, 100 mM NaCl, 3 mM EDTA, 0.05% BSA, 0.1% Tween20. Prior to reading, Streptavidin allophycocyanin (SA-APC) is added at a final concentration in the assay of 0.0004 mg/mL, along with europilated anti-phosphotyrosine Ab (Eu-anti-PY) at a final conc of 0.025 nM. The assay plate is read in a Discovery fluorescence plate reader with excitation at 320 nm and emission at 615 and 655 nm[1].
Synonyms	KX1004, KX1 004

Chemical Properties

Molecular Weight	284.29
Formula	C₁₆H₁₃FN₂O₂
Cas No.	518058-84-9
Smiles	OC1=CC=CC(CNC(=O)C2=CC3=CC(F)=CC=C3N2)=C1
Relative Density.	1.382 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (211.05 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.04 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.5175 mL	17.5877 mL	35.1753 mL	175.8767 mL
5 mM	0.7035 mL	3.5175 mL	7.0351 mL	35.1753 mL
10 mM	0.3518 mL	1.7588 mL	3.5175 mL	17.5877 mL
20 mM	0.1759 mL	0.8794 mL	1.7588 mL	8.7938 mL
50 mM	0.0704 mL	0.3518 mL	0.7035 mL	3.5175 mL
100 mM	0.0352 mL	0.1759 mL	0.3518 mL	1.7588 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc