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Ionomycin

Name: Ionomycin
Brand: TargetMol
SKU: T7285
Price: 196 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7285Cas No. 56092-81-0

Ionomycin is a calcium ion carrier and an antibiotic that binds to calcium ions (Ca2+). The main function of Ionomycin is to induce cellular responses by increasing the intracellular calcium ion concentration. In experiments, Ionomycin is typically used to activate calcium-dependent processes, such as apoptosis and enzyme activity.

Ionomycin

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Purity: 99.54%

Catalog No. T7285Cas No. 56092-81-0

Pack Size	Price	Availability	Quantity
1 mg	$196	In Stock

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Purity:99.54%

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Ionomycin is a calcium ion carrier and an antibiotic that binds to calcium ions (Ca2+). The main function of Ionomycin is to induce cellular responses by increasing the intracellular calcium ion concentration. In experiments, Ionomycin is typically used to activate calcium-dependent processes, such as apoptosis and enzyme activity.
In vitro	METHODS: LCLC 103H cells were treated with Ionomycin and analyzed using DNA and protein. RESULTS: Ionomycin induces DNA fragmentation and PARP lysis into an 85-kDa fragment of typical caspase-mediated apoptosis. [1]
In vivo	METHODS: To investigate the activation of immune cells by Ionomycin, PMA and Ionomycin were used to activate CD4+CD25- T cells in mice to generate inductive regulatory T cells (iTreg), and their functions were tested in a mouse model of inflammatory bowel disease (IBD). RESULTS: iTreg cells activated by Ionomycin could significantly alleviate the symptoms of IBD mouse models, including weight loss and reduced disease activity scores. [2] METHODS: To study the role of calcium signaling in embryonic development, Ionomycin was added to the mouse embryo culture medium. RESULTS: Calcium signaling plays a key role in embryonic cell migration and morphogenesis. [3]

Chemical Properties

Molecular Weight	709.01
Formula	C₄₁H₇₂O₉
Cas No.	56092-81-0
Smiles	[H][C@@]1(C[C@H](O)[C@H](C)[C@H](O)[C@H](C)\C=C\C[C@@H](C)C[C@@H](C)C(\O)=C\C(=O)[C@@H](C)C[C@@H](C)C[C@H](C)CCC(O)=O)CC[C@](C)(O1)[C@@]1([H])CC[C@](C)(O1)[C@@H](C)O
Relative Density.	1.072 g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 90.0 mg/mL (126.9 mM), Sonication is recommended.

DMSO: 90.0 mg/mL (126.9 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (4.65 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4104 mL	7.0521 mL	14.1042 mL	70.5209 mL
5 mM	0.2821 mL	1.4104 mL	2.8208 mL	14.1042 mL
10 mM	0.1410 mL	0.7052 mL	1.4104 mL	7.0521 mL
20 mM	0.0705 mL	0.3526 mL	0.7052 mL	3.5260 mL
50 mM	0.0282 mL	0.1410 mL	0.2821 mL	1.4104 mL
100 mM	0.0141 mL	0.0705 mL	0.1410 mL	0.7052 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc