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Ionomycin

Name: Ionomycin
Brand: TargetMol
SKU: T7285
Price: 196 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7285Cas No. 56092-81-0

Ionomycin is a calcium ion carrier and an antibiotic that binds to calcium ions (Ca2+). The main function of Ionomycin is to induce cellular responses by increasing the intracellular calcium ion concentration. In experiments, Ionomycin is typically used to activate calcium-dependent processes, such as apoptosis and enzyme activity.

Ionomycin

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Purity: 99.54%

Catalog No. T7285Cas No. 56092-81-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$196	In Stock	-

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.54%

Color:White

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Ionomycin is a calcium ion carrier and an antibiotic that binds to calcium ions (Ca2+). The main function of Ionomycin is to induce cellular responses by increasing the intracellular calcium ion concentration. In experiments, Ionomycin is typically used to activate calcium-dependent processes, such as apoptosis and enzyme activity.
In vitro	METHODS: LCLC 103H cells were treated with Ionomycin and analyzed using DNA and protein. RESULTS: Ionomycin induces DNA fragmentation and PARP lysis into an 85-kDa fragment of typical caspase-mediated apoptosis. [1]
In vivo	METHODS: To investigate the activation of immune cells by Ionomycin, PMA and Ionomycin were used to activate CD4+CD25- T cells in mice to generate inductive regulatory T cells (iTreg), and their functions were tested in a mouse model of inflammatory bowel disease (IBD). RESULTS: iTreg cells activated by Ionomycin could significantly alleviate the symptoms of IBD mouse models, including weight loss and reduced disease activity scores. [2] METHODS: To study the role of calcium signaling in embryonic development, Ionomycin was added to the mouse embryo culture medium. RESULTS: Calcium signaling plays a key role in embryonic cell migration and morphogenesis. [3]

Chemical Properties

Molecular Weight	709.01
Formula	C₄₁H₇₂O₉
Cas No.	56092-81-0
Smiles	[H][C@@]1(C[C@H](O)[C@H](C)[C@H](O)[C@H](C)\C=C\C[C@@H](C)C[C@@H](C)C(\O)=C\C(=O)[C@@H](C)C[C@@H](C)C[C@H](C)CCC(O)=O)CC[C@](C)(O1)[C@@]1([H])CC[C@](C)(O1)[C@@H](C)O
Relative Density.	1.072 g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 90 mg/mL (126.94 mM), Sonication is recommended.

DMSO: 90 mg/mL (126.94 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (4.65 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4104 mL	7.0521 mL	14.1042 mL	70.5209 mL
5 mM	0.2821 mL	1.4104 mL	2.8208 mL	14.1042 mL
10 mM	0.1410 mL	0.7052 mL	1.4104 mL	7.0521 mL
20 mM	0.0705 mL	0.3526 mL	0.7052 mL	3.5260 mL
50 mM	0.0282 mL	0.1410 mL	0.2821 mL	1.4104 mL
100 mM	0.0141 mL	0.0705 mL	0.1410 mL	0.7052 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc