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HA Peptide

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Catalog No. TP1276Cas No. 92000-76-5

Influenza Hemagglutinin (HA) Peptide is a tag peptide derived from an epitope of the influenza hemagglutinin protein.

HA Peptide

HA Peptide

🥰Excellent
Catalog No. TP1276Cas No. 92000-76-5
Influenza Hemagglutinin (HA) Peptide is a tag peptide derived from an epitope of the influenza hemagglutinin protein.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$47In StockIn Stock
2 mg$57In StockIn Stock
5 mg$77In StockIn Stock
10 mg$137In StockIn Stock
25 mg$228In StockIn Stock
50 mg$343In StockIn Stock
100 mg$497In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:>99.99%
Appearance:Solid
Color:White
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Product Introduction

Bioactivity
Description
Influenza Hemagglutinin (HA) Peptide is a tag peptide derived from an epitope of the influenza hemagglutinin protein.
In vitro
HA Peptide, derived from human influenza hemagglutinin (HA) amino acids 98–106, is a potent epitope widely utilized for isolating, purifying, detecting, and tracking proteins of interest due to its strong immunoreactivity. It is commonly employed in the separation of tagged proteins from cell culture supernatants and lysates at neutral pH, making it a valuable tool for coimmunoprecipitation and detection via western blotting. Given its small size, HA Peptide minimally impacts the biological activity and functionality of fusion partner proteins. The purification of recombinant HA-tagged proteins is facilitated by a highly specific anti-HA monoclonal antibody immobilized on resin, allowing for separation. These proteins can be eluted using a gentle method with an HA epitope at 1 mg/mL in TBS or through one of the three chemical elutions: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH. Additionally, incorporating an N-terminal HA Peptide into mammalian expression vectors is crucial for T7 promoter-driven expression in E. coli, independent of trans-acting T7 RNAP. However, research indicates that caspase 3/7-mediated cleavage of HA Peptide abolishes its immunoreactivity, cautioning against its use in studying cell death and apoptotic mechanisms due to potential artifacts.
Chemical Properties
Molecular Weight1102.15
FormulaC53H67N9O17
Cas No.92000-76-5
SmilesCC(C)C(NC(=O)C(CC(O)=O)NC(=O)C(Cc1ccc(O)cc1)NC(=O)C1CCCN1C(=O)C(N)Cc1ccc(O)cc1)C(=O)N1CCCC1C(=O)NC(CC(O)=O)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(C)C(O)=O
Relative Density.1.414 g/cm3 at 20℃
SequenceTyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala
Sequence ShortYPYDVPDYA
Storage & Solubility Information
Storagekeep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: 65 mg/mL (58.98 mM)
DMSO: 10 mM, Sonication is recommended.
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM0.9073 mL4.5366 mL9.0732 mL45.3659 mL
5 mM0.1815 mL0.9073 mL1.8146 mL9.0732 mL
10 mM0.0907 mL0.4537 mL0.9073 mL4.5366 mL
H2O
1mg5mg10mg50mg
20 mM0.0454 mL0.2268 mL0.4537 mL2.2683 mL
50 mM0.0181 mL0.0907 mL0.1815 mL0.9073 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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