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Gap 27

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Catalog No. TP1333Cas No. 198284-64-9

Gap 27 is a peptide(Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile) derived from connexin 43 that is a selective gap junction blocker.

Gap 27

Gap 27

🥰Excellent
Catalog No. TP1333Cas No. 198284-64-9
Gap 27 is a peptide(Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile) derived from connexin 43 that is a selective gap junction blocker.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$48In StockIn Stock
5 mg$97In StockIn Stock
10 mg$141In StockIn Stock
25 mg$236In StockIn Stock
50 mg$352In StockIn Stock
100 mg$528In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:>99.99%
Appearance:solid
Color:White
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Product Introduction

Bioactivity
Description
Gap 27 is a peptide(Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile) derived from connexin 43 that is a selective gap junction blocker.
In vitro
Gap 27 significantly reduces the population of TRAP-positive mononuclear and multinucleated rat osteoclasts on bovine bone slices and decreases the functionality of remaining osteoclasts, as demonstrated by a lower percentage of osteoclasts displaying actin rings among all TRAP-positive cells. Moreover, the area resorbed by these treated cultures is substantially reduced[1]. When applied to the carotid artery, Gap 27 at a concentration of 500 μM effectively eliminates the hyperpolarization response to acetylcholine, but does not affect the response to levcromakalim, indicating a specific inhibition of acetylcholine-induced, endothelium-dependent hyperpolarizations in the guinea-pig-isolated internal carotid artery[2].
In vivo
Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta but did not modify force development in response to PhE[3].
Chemical Properties
Molecular Weight1304.53
FormulaC60H101N15O17
Cas No.198284-64-9
SmilesC([C@@H](NC([C@H](CO)N)=O)CCCNC(=N)N)(=O)N1[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CC2=CC=CC=C2)C(N[C@H](C(N[C@@H]([C@H](CC)C)C(O)=O)=O)[C@H](CC)C)=O)=O)[C@H](CC)C)=O)[C@@H](C)O)=O)CCCCN)=O)CCC(O)=O)=O)[C@@H](C)O)=O)CCC1
Relative Density.1.40 g/cm3 (Predicted)
SequenceSer-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile
Sequence ShortSRPTEKTIFII
Storage & Solubility Information
Storagekeep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: Insoluble
H2O: 10 mM, Sonication is recommended.
Solution Preparation Table
H2O
1mg5mg10mg50mg
1 mM0.7666 mL3.8328 mL7.6656 mL38.3280 mL
5 mM0.1533 mL0.7666 mL1.5331 mL7.6656 mL
10 mM0.0767 mL0.3833 mL0.7666 mL3.8328 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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μL
2 Enter the in vivo formulation:
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%
% Tween 80
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitGapJunctionProteinGap-27Gap27Gap Junction ProteinGap 27
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