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Folinic acid

Name: Folinic acid
Brand: TargetMol
SKU: T24403
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T24403Cas No. 58-05-9

Alias Leucovorin, HSDB 6544

Folinic acid (Leucovorin) is a biofolate used in the treatment of methotrexate (MTX) toxicity reduction and as an adjuvant therapy for colon cancer.

Folinic acid

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Purity: 99.76%

Catalog No. T24403Alias Leucovorin, HSDB 6544Cas No. 58-05-9

Folinic acid (Leucovorin) is a biofolate used in the treatment of methotrexate (MTX) toxicity reduction and as an adjuvant therapy for colon cancer.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$33	In Stock	In Stock
25 mg	$50	In Stock	In Stock
50 mg	$70	In Stock	In Stock
100 mg	$100	In Stock	In Stock
500 mg	$239	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.76%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Folinic acid (Leucovorin) is a biofolate used in the treatment of methotrexate (MTX) toxicity reduction and as an adjuvant therapy for colon cancer.
In vitro	Methotrexate (MTX) alone induces a concentration-dependent increase in the percentage of micronucleated binucleated cells (MNBN) and abnormal cells (Abs). As the concentration of MTX increases, the nuclear division index (NDI) decreases. Similarly, the mitotic index (MI) also decreases at all tested concentrations of MTX. The addition of 50 μg/mL folinic acid significantly reduces the % MNBN (40-68%) and % Abs (36-77%). In the presence of 5 μg/mL folinic acid, inhibitory effects are observed as well (MNBN from 12% to 54%, Abs from 20% to 61%)[2].
In vivo	Treatment with folinic acid (7.0 mg/kg; intraperitoneal injection; every other day; for 3 weeks) appears to reverse the growth inhibition induced by Methotrexate (MTX) administration in young male Balb/c mice. Long-term MTX administration is known to inhibit skeletal growth in mice[1].
Synonyms	Leucovorin, HSDB 6544

Chemical Properties

Molecular Weight	473.44
Formula	C₂₀H₂₃N₇O₇
Cas No.	58-05-9
Smiles	Nc1nc2NCC(CNc3ccc(cc3)C(=O)NC(CCC(O)=O)C(O)=O)N(C=O)c2c(=O)[nH]1
Relative Density.	1.4485 g/cm3 (Estimated)

Storage & Solubility Information

Storage

keep away from direct sunlight,The compound is unstable in solution. Please use soon | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 260 mg/mL (549.17 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.22 mM), The compound is unstable in solution, please use soon.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1122 mL	10.5610 mL	21.1220 mL	105.6100 mL
5 mM	0.4224 mL	2.1122 mL	4.2244 mL	21.1220 mL
10 mM	0.2112 mL	1.0561 mL	2.1122 mL	10.5610 mL
20 mM	0.1056 mL	0.5281 mL	1.0561 mL	5.2805 mL
50 mM	0.0422 mL	0.2112 mL	0.4224 mL	2.1122 mL
100 mM	0.0211 mL	0.1056 mL	0.2112 mL	1.0561 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc