Dansylcadaverine (Monodansyl cadaverine) is a fluorescent probe.

The amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin.

Dansylcadaverine staining
a. Solution preparation:
1. Mother solution preparation: Prepare a certain concentration of Dansylcadaverine mother solution, store it at -20℃ or -80℃ in the dark after aliquoting.
2. Working solution preparation: Select the appropriate working solution concentration according to the experimental requirements, and try to prepare it before use.
b. Operation steps:
1. Inoculate cells in a 6-well plate (or 12-well plate) at a concentration of 3x104 cells/well, and culture in a 5% CO2 incubator at 37°C.
2. Add 50 mM Dansylcadaverine working solution to each well and incubate for 15 minutes.
3. Rinse the cells 3 times with PBS and observe the staining under a microscope.