Biotin-aniline is a probe that reacts strongly to RNA and DNA.Biotin-aniline is highly efficient and is used to capture subcellular transcriptomes in living cells with high spatial specificity.

1. Subcellular transcriptome capture
Experimental steps:
1. Prepare a solution containing Biotin-aniline, which is usually dissolved in a suitable solvent (such as water or PBS).
2. Add Biotin-aniline solution to live cell culture medium and incubate for 1-2 hours under the conditions of cell growth so that Biotin-aniline can enter the cells and bind to RNA.
3. After incubation, capture and purification using avidin or avidin to isolate the target RNA.
4. Extract and analyze the target RNA and use RT-PCR, RNA-seq and other technologies for transcriptome analysis.
2. RNA/DNA marker
Experimental steps
1. Prepare an appropriate amount of Biotin-aniline and dissolve it in a suitable solvent.
2. Add Biotin-aniline to the target RNA or DNA sample to react with the nucleic acid.
3. Use avidin or streptavidin for labeled molecular capture.
4. Analysis is carried out through appropriate detection methods (such as fluorescence microscopy or molecular detection instrument).
3. Real-time analysis of living cells
Experimental steps:
1. Add Biotin-aniline solution to live cell culture medium.
2. Incubate for a certain period of time (usually 1-2 hours) under cell culture conditions.
3. Use appropriate fluorescently labeled antibodies or avidin to perform fluorescence imaging to observe the distribution and dynamics of RNA in the cells.