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Thiazovivin

Catalog No. T2155 CAS 1226056-71-8

Thiazovivin, a ROCK inhibitor (IC50: 0.5 μM), increases the survival rate of hESC.

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Thiazovivin, CAS 1226056-71-8

Pack Size	Availability	Price/USD
1 mg	In stock	$ 30.00
2 mg	In stock	$ 44.00
5 mg	In stock	$ 68.00
10 mg	In stock	$ 117.00
25 mg	In stock	$ 222.00
50 mg	In stock	$ 401.00
100 mg	In stock	$ 597.00
1 mL * 10 mM (in DMSO)	In stock	$ 75.00

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Purity: 98.75%

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Description	Thiazovivin, a ROCK inhibitor (IC50: 0.5 μM), increases the survival rate of hESC.
Targets&IC50	ROCK:~0.5 μM （cell free)
In vitro	When the cells were cultured in the presence of an E-cadherin blocking antibody, the formation of large, compact aggregates following Thiazovivin treatment was severely inhibited and extensive cell death was observed. Thiazovivin (2 μM) inhibits ROCK activity and protects hESCs at a similar level as Y-27632 (10 μM), a widely used selective ROCK inhibitor [1]. Attachment rates of blastocyst and embryonic cell clumps onto feeder cells in the Thiazovivin treatment group were greater than those of the control group. The pluripotency markers of the OCT4 and NANOG genes and the adhesion molecule E-cadherin were increased by Thiazovivin treatment [2]. By adding thiazovivin to reprogramming cultures, the reprogramming efficiency of CB cells increases by more than 10 times [3].
Kinase Assay	Synthesis of compound immobilized affinity matrixes: Compounds Tzv and its inactive analog (10 mg each) in DMSO (500 μL) and Et3N (10.4 μL) were added to Reacti-Gel (0.5 mL, 25 μmol) that was washed by DMSO in an Eppendorf vial. The reaction mixture was incubated at room temperature until the starting material disappeared (determined by HPLC). After the disappearance of the starting material, ethanolamine (15 μL) was added and the resulting mixture was incubated at room temperature overnight to block the Reacti-Gel. The resulting affinity matrices were washed thoroughly with DMSO (500 μL × 4), PBS (500 μL × 2), and stored at 4 °C in NaN3 solution (0.1% in PBS). Affinity pull-down was performed as described previously. Briefly, whole cell lysates were pretreated with the unfunctionalized affinity matrix at 4 °C for 1.5 h. After washing three times, samples were incubated with the positive or negative affinity matrix at 4 °C for 1 h. After heat shock, samples were loaded and separated on a 4–20% Tris-Glycine SDS PAGE and silver stained with a Silver Stain Plus Kit. The differentially retained protein bands were cut, destained, and analyzed with LCMS [1].
Cell Research	Chemically defined and feeder-free human embryonic stem cell (hESC) culture was described briefly as following. hESCs were grown on Matrigel-coated tissue culture plates in N2B27-CDM [DMEM-F12 supplemented with 1× N2 supplements, 1× B27 supplements, 2 mM L glutamine, 0.11 mM 2-mercaptoethanol, 1× nonessential amino acids, and 0.5 mg/mL BSA (fraction V)] and 20 ng/mL bFGF. Human ESCs were passaged every five to six days with 0.05% trypsin. Murine ESCs are cultured in knockout DMEM supplement with 2 mM L glutamine, 1× nonessential amino acids, 15% serum replacement, and 1 × 10^3 ng/mL leukemia inhibitory factor (LIF). For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For low-density survival assays, 500 cells were plated onto 96-well Matrigel-coated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity as described in the manufacturer's instructions. ALP-positive colonies were counted on an inverted microscope. For growing hESCs in mouse medium, HES2, HUES7, HUES9, and HUES1-Oct4-GFP were cultured in murine ESC (mESC) growth media supplemented with 1-μM mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor PD0325901 and 5-μM p38 inhibitor SB202190 and 1 × 10^3 human LIF [1].

Molecular Weight	311.36
Formula	C15H13N5OS
CAS No.	1226056-71-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 31.1 mg/mL (100 mM)

References and Literature

1. Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34. 2. Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57. 3. Hu K, et al. Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells. Blood. 2011 Apr 7;117(14):e109-19.

Citations

1. Ma X, Lu Y, Zhou Z, Human expandable pancreatic progenitor–derived β cells ameliorate diabetes. Science Advances. 2022, 8(8): eabk1826.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library TGF-beta/Smad Compound Library Bioactive Compound Library Kinase Inhibitor Library Anti-Aging Compound Library Cytoskeletal Signaling Pathway Compound Library Neural Regeneration Compound Library Anti-Metabolism Disease Compound Library NO PAINS Compound Library Anti-Cancer Compound Library

Related Products

Related compounds with same targets

CCG-222740 Belumosudil Y-27632 SAR407899 RKI-1447 Verosudil Chroman 1 ROCK2-IN-7

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Keywords

Thiazovivin 1226056-71-8 Cell Cycle/Checkpoint Cytoskeletal Signaling Stem Cells ROCK Inhibitor Rho-associated protein kinase ROK Rho-associated kinase inhibit Rho-kinase inhibitor

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