Powder: -20°C for 3 years | In solvent: -80°C for 1 year
N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 39.00 | |
25 mg | In stock | $ 80.00 | |
50 mg | In stock | $ 128.00 | |
100 mg | In stock | $ 193.00 | |
500 mg | In stock | $ 479.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 30.00 |
Description | N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA. |
In vitro | In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1]. |
In vivo | mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2]. |
Synonyms | 1-Methylpseudouridine |
Molecular Weight | 258.23 |
Formula | C10H14N2O6 |
CAS No. | 13860-38-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 40 mg/mL (154.90 mM)
DMSO: 100 mg/mL (387.25 mM)
You can also refer to dose conversion for different animals. More
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N1-Methylpseudouridine 13860-38-3 Others GFP Inhibitor mRNA inhibit DNA/RNA Synthesis eIF2α Nucleoside Antimetabolite/Analog phosphorylation protein expression N1 Methylpseudouridine N-1-Methylpseudouridine cells HEK293T 1-Methylpseudouridine polysomes ribosome N1Methylpseudouridine inhibitor