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N1-Methylpseudouridine

N1-Methylpseudouridine
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Purity:100%
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N1-Methylpseudouridine

Catalog No. T19459Cas No. 13860-38-3
N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.
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Pack SizePrice/USDAvailabilityQuantity
10 mg$39In Stock
25 mg$80In Stock
50 mg$128In Stock
100 mg$193In Stock
500 mg$479In Stock
1 mL x 10 mM (in DMSO)$30In Stock
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Related Compound Libraries of "N1-Methylpseudouridine"

Product Introduction

Bioactivity
Description
N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.
In vitro
In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1].
In vivo
mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2].
Alias1-Methylpseudouridine
Chemical Properties
Molecular Weight258.23
FormulaC10H14N2O6
Cas No.13860-38-3
Storage & Solubility Information
Storage Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
H2O: 40 mg/mL (154.90 mM)
DMSO: 100 mg/mL (387.25 mM)
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM3.8725 mL19.3626 mL38.7252 mL193.6258 mL
5 mM0.7745 mL3.8725 mL7.7450 mL38.7252 mL
10 mM0.3873 mL1.9363 mL3.8725 mL19.3626 mL
20 mM0.1936 mL0.9681 mL1.9363 mL9.6813 mL
50 mM0.0775 mL0.3873 mL0.7745 mL3.8725 mL
100 mM0.0387 mL0.1936 mL0.3873 mL1.9363 mL

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