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N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride

Catalog No. T16169 CAS 4272-74-6

Synonyms: N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride is an inhibitor of trypsin-like Protease. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride exhibits an inhibitory effect on IFN-γ activities.

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N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride, CAS 4272-74-6

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride is an inhibitor of trypsin-like protease. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride exhibits an inhibitory effect on IFN-γ activities.

In vitro

A lower concentration of anti-Fas (10 ng/mL) is used to examine the interaction among the three effectors simultaneously, that is, anti-Fas, TLCK, and IFN-γ. N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride (50 μM) exhibits a small decrease in cell viability. Beyond 50 μM, a dose-dependent decrease in cell viability is observed. IFN-γ slightly reduces cell viability on its own. The addition of anti-Fas (10 ng/mL) results in a slight decrease in cell survival, which is enhanced more than additively in the presence of TLCK, most prominently between 50 and 100 μM. Upon addition of both anti-Fas and IFN-γ, a decrease (≈46%) in cell viability is observed. Moreover, the decrease in cell survival is further enhanced by the addition of higher concentrations of TLCK, 25 μM, and more[1].

Synonyms	N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride
Molecular Weight	369.31
Formula	C14H22Cl2N2O3S
CAS No.	4272-74-6

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 95 mg/mL(257.24 mM), Need ultrasonic

DMSO: 245 mg/mL (663.40 mM), Need ultrasonic

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Shadrin N, et al. Serine protease inhibitors interact with IFN-γ through up-regulation of FasR; a novel therapeutic strategy against cancer. Exp Cell Res. 2015 Jan 15;330(2):233-9.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

N-alpha-Tosyl-L-lysine chloromethyl ketone hydrochloride 4272-74-6 Immunology/Inflammation Neuroscience Others Proteases/Proteasome Ubiquitination COX Proteasome Inhibitor inhibit N alpha Tosyl L lysine chloromethyl ketone hydrochloride NalphaTosylLlysine chloromethyl ketone hydrochloride N-alpha-Tosyl-L-lysine_chloromethyl_ketone_hydrochloride inhibitor

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