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Levoleucovorin Calcium

Catalog No. T6431 CAS 80433-71-2

Synonyms: Calcium Levofolinate, CL307782

Levoleucovorin Calcium (CL307782), a calcium salt of the folinic acid, is used in cancer chemotherapy as an adjuvant.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Levoleucovorin Calcium Chemical Structure

Levoleucovorin Calcium, CAS 80433-71-2

Pack Size	Availability	Price/USD
50 mg	In stock	$ 36.00
100 mg	In stock	$ 48.00
500 mg	In stock	$ 63.00

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Purity: 99.68%

Purity: 98.46%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Levoleucovorin Calcium (CL307782), a calcium salt of the folinic acid, is used in cancer chemotherapy as an adjuvant.

Kinase Assay

Chk1 Assay: For the Chk1 assay, the kinase domain is expressed in Sf9 insect cells, and a biotinylated cdc25c peptide containing the consensus Chk1/Chk2 phosphorylation site (*)(biotin-[AHX]SGSGS*GLYRSPSMP-ENLNRPR[CONH2]) is used as the substrate. A dilution series of CHIR-124 is mixed with a kinase reaction buffer containing a final concentration of 30 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM DTT, 4 mM EDTA, 25 mMβ-glycerophosphate, 5 mM MnCl2, 0.01% bovine serum albumin, 1.35 nM CHK1 kinase domain, 0.5 μM peptide substrate, and 1 AM unlabeled ATP, plus 5 nM 33Pγ-labeled ATP (specific activity = 2,000 Ci/mmol). Reactions and detection of the phosphate transfer are carried out by a radioactive method. Reactions are incubated at room temperature for 1 to 4 hours and the phosphorylated peptide captured on streptavidin-coated microtiter plates containing stop reaction buffer (25 mM EDTA [ethylenediaminetetraacetic acid], 50 mMHEPES, pH 7.5). Phosphorylated peptide is measured with the DELFIA TRF system using a Europium-labeled anti-phosphotyrosine antibody PT66. The concentration of CHIR-124 for IC50 is calculated using nonlinear regression with XL-Fit data analysis software.

Synonyms	Calcium Levofolinate, CL307782
Molecular Weight	511.5
Formula	C20H21N7O7·Ca
CAS No.	80433-71-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: Insoluble, The compound is unstable in solution, please use soon.

H2O: 10 mM

References and Literature

1. Lebitasy M, Hecq JD, Athanassopoulos A, Vanbeckbergen D, Jamart J, Galanti L.J Clin Pharm Ther. 2009 Aug;34(4):423-8. 2. Honma H.Gan To Kagaku Ryoho. 2006 Nov;33(11):1633-6. Japanese.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Tumor Natural Product Library Anti-Colorectal Cancer Compound Library Covalent Inhibitor Library Drug-induced Liver Injury (DILI) Compound Library Toxic Compound Library

Related Products

Related compounds with same targets

Folinic acid Folinic Acid Calcium Salt Pentahydrate Proguanil hydrochloride Pyrimethamine Trimethoprim Pemetrexed disodium Methotrexate Folinic acid calcium

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Levoleucovorin Calcium 80433-71-2 Cell Cycle/Checkpoint Others Antifolate CL-307782 inhibit anticancer Calcium Levofolinate CL 307782 5-Fluorouracil antineoplastic Levoleucovorin Inhibitor CL307782 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.