Powder: -20°C for 3 years | In solvent: -80°C for 1 year
IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 43.00 | |
10 mg | In stock | $ 61.00 | |
25 mg | In stock | $ 113.00 | |
50 mg | In stock | $ 210.00 | |
100 mg | In stock | $ 315.00 | |
200 mg | In stock | $ 473.00 | |
500 mg | In stock | $ 776.00 | |
1 mL * 2.5 mM (in DMSO) | In stock | $ 57.00 |
Description | IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation. |
Targets&IC50 | Wnt:27 nM |
In vivo | To assess IWP-2's effectiveness in vivo, 200 μL of IWP-2-liposome or free liposome was administered intraperitoneally to C57BL/6 mice approximately 2 hours before they received a similar volume of blue-dye-filled latex beads or E. coli DH5α. IWP-2 significantly diminished both blue bead and E. coli uptake, evidenced by reduced colony-forming units (CFUs) in peritoneal lavage cells within 2 hours. Concurrently, IWP-2 treatment led to a 2-4-fold decrease in TNF-α and IL-6 levels in the lavage fluid, while notably increasing IL-10 secretion, an anti-inflammatory cytokine. Moreover, pretreating with IWP-2 markedly negated the Substance P (SP)-induced upregulation of Wnt3a, p-GSK3β, and β-catenin expressions, demonstrating its potential in modulating inflammatory responses and Wnt signaling pathways. |
Cell Research | IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera[2]. |
Synonyms | IWP2 |
Molecular Weight | 466.6 |
Formula | C22H18N4O2S3 |
CAS No. | 686770-61-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 2 mg/mL(4.29mM)
You can also refer to dose conversion for different animals. More
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IWP-2 686770-61-6 Cytoskeletal Signaling Metabolism Stem Cells Casein Kinase Wnt/beta-catenin Porcupine Wnt Inhibitor IWP 2 IWP2 inhibit inhibitor