Home Tools
Log in
Cart

IWP-2

Catalog No. T2702   CAS 686770-61-6
Synonyms: IWP2

IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
IWP-2 Chemical Structure
IWP-2, CAS 686770-61-6
Pack Size Availability Price/USD Quantity
5 mg In stock $ 43.00
10 mg In stock $ 61.00
25 mg In stock $ 113.00
50 mg In stock $ 210.00
100 mg In stock $ 315.00
200 mg In stock $ 473.00
500 mg In stock $ 776.00
1 mL * 2.5 mM (in DMSO) In stock $ 57.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 99.76%
Purity: 96.1%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description IWP-2, an inhibitor of Wnt processing and secretion (IC50=27 nM) in a cell-free assay, selectively blocks Porcn-mediated Wnt palmitoylation.
Targets&IC50 Wnt:27 nM
In vivo To assess IWP-2's effectiveness in vivo, 200 μL of IWP-2-liposome or free liposome was administered intraperitoneally to C57BL/6 mice approximately 2 hours before they received a similar volume of blue-dye-filled latex beads or E. coli DH5α. IWP-2 significantly diminished both blue bead and E. coli uptake, evidenced by reduced colony-forming units (CFUs) in peritoneal lavage cells within 2 hours. Concurrently, IWP-2 treatment led to a 2-4-fold decrease in TNF-α and IL-6 levels in the lavage fluid, while notably increasing IL-10 secretion, an anti-inflammatory cytokine. Moreover, pretreating with IWP-2 markedly negated the Substance P (SP)-induced upregulation of Wnt3a, p-GSK3β, and β-catenin expressions, demonstrating its potential in modulating inflammatory responses and Wnt signaling pathways.
Cell Research IWP-2 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. The human RCC cell lines 786O and Caki-2 (5×103) are seeded into 96-well plates. Cell viability is estimated by MST assay after Caki-2 acells are incubated with ncreasing concentrations of LEF together with 20 μM IWP-2 for 48 h.After treatment, 10 μL MTS is added into each well for 2 h incubation. The absorbance is measured using a model ELX800 Micro Plate Reader at 490 nm. For colony formation assay, Caki-2 cells are trypsinized to single cell suspensions and seeded into fresh 6-well plates at 1000 cells/well. Then cells are incubated with LEF at depicted concentrations for 7 days. Colonies are fixed with absolute methanol for 15 min and then stained with 0.1% crystal violet for 20 min. After washing with PBS three times, the colonies with a diameter over 2 mm are visualized by a digital camera[2].
Synonyms IWP2
Molecular Weight 466.6
Formula C22H18N4O2S3
CAS No. 686770-61-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 2 mg/mL(4.29mM)

TargetMolReferences and Literature

1. Chen B, et al. Nat Chem Biol, 2009, 5(2), 100-107. 2. Maiti G, et al. The Wingless homolog Wnt5a stimulates phagocytosis but not bacterial killing. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16600-5. 3. Liu JD, et al. Wnt/Glycogen Synthase Kinase 3β/β-catenin Signaling Activation Mediated Sevoflurane Preconditioning-induced Cardioprotection. Chin Med J (Engl). 2015 Sep 5;128(17):2346-53.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Wnt/Hedgehog/Notch Compound Library Neuronal Differentiation Compound Library Reprogramming Compound Library Anti-Colorectal Cancer Compound Library Anti-Lung Cancer Compound Library Target-Focused Phenotypic Screening Library Kinase Inhibitor Library Anti-Cancer Compound Library Neural Regeneration Compound Library

Related Products

Related compounds with same targets
K-756 Specnuezhenide CHIR-99021 DK419 Trachelogenin Nefopam hydrochloride 4,10-Aromadendranediol CCT251545

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

IWP-2 686770-61-6 Cytoskeletal Signaling Metabolism Stem Cells Casein Kinase Wnt/beta-catenin Porcupine Wnt Inhibitor IWP 2 IWP2 inhibit inhibitor

 

TargetMol