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DPPH

Name: DPPH
Brand: TargetMol
SKU: T11093
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11093Cas No. 1898-66-4

Alias 2,2-Diphenyl-1-picrylhydrazyl

DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a cell-permeable, stable free radical commonly used to evaluate the free radical scavenging ability, hydrogen donation capacity of compounds, and to measure the antioxidant activity of tissue extracts.

DPPH

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Purity: 99.71%

Catalog No. T11093Alias 2,2-Diphenyl-1-picrylhydrazylCas No. 1898-66-4

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$30	In Stock	In Stock
50 mg	$40	In Stock	In Stock
100 mg	$62	In Stock	In Stock
500 mg	$150	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.71%

Appearance:Solid

Color:Black

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COA LCMS

Product Introduction

Bioactivity

Description	DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a cell-permeable, stable free radical commonly used to evaluate the free radical scavenging ability, hydrogen donation capacity of compounds, and to measure the antioxidant activity of tissue extracts.
Targets&IC50	PNT2 cells:1400 µM
In vitro	DPPH shows a strong absorption band at 517 nm due to its odd electron number, resulting in a dark purple solution that loses color as electron pairs detach, with decoloration stoichiometrically depending on the absorbed electrons. In alcohol solutions of 0.5 mM, it adheres to the Lambert-Beer law within the effective absorption range. DPPH is a fast, simple, and cost-effective method to evaluate the free radical scavenging or hydrogen-donating ability of compounds and the antioxidant activity of foods. It can quantify antioxidants in complex biological systems and solid or liquid samples. This method effectively determines the total antioxidant capacity and free radical scavenging activity in fruit and vegetable juices, wheat grains and bran, vegetables, conjugated linoleic acid, herbal medicine, edible seed oil, and flour in various solvents (ethanol, acetone, methanol, ethanol, and benzene). It also assesses antioxidant properties in cysteine, glutathione, ascorbic acid, tocopherol, and polyhydroxyaromatic compounds in olive oil, fruits, fruit juices, and wines.
In vivo	METHODS: To study the hepatotoxic effects, DPPH (100 mg/kg) was administered intraperitoneally to ICR mice to induce acute liver injury, and the mice were killed 12, 24, 48 and 72 h after treatment to obtain serum and liver. RESULTS: DPPH increased aminotransferase activity, MDA and NO levels, and significantly decreased GSH levels and SOD, CAT and GPx activities.The livers of DPPH-treated mice showed significant histopathological changes characterized by hepatocyte swelling and vacuolar degeneration.The mRNA levels of proinflammatory cytokines were elevated in the DPPH-treated group. The above results suggest that DPPH induces acute hepatotoxicity in mice. [3]
Synonyms	2,2-Diphenyl-1-picrylhydrazyl

Chemical Properties

Molecular Weight	394.32
Formula	C₁₈H₁₂N₅O₆
Cas No.	1898-66-4
Smiles	[O-][N+](=O)c1cc(c(NN(c2ccccc2)c2ccccc2)c(c1)[N+]([O-])=O)[N+]([O-])=O
Relative Density.	1.484 g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 24 mg/mL (60.86 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.07 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5360 mL	12.6801 mL	25.3601 mL	126.8006 mL
5 mM	0.5072 mL	2.5360 mL	5.0720 mL	25.3601 mL
10 mM	0.2536 mL	1.2680 mL	2.5360 mL	12.6801 mL
20 mM	0.1268 mL	0.6340 mL	1.2680 mL	6.3400 mL
50 mM	0.0507 mL	0.2536 mL	0.5072 mL	2.5360 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc