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DMPO

Catalog No. T22739 CAS 3317-61-1

Synonyms: 5,5-Dimethyl-1-pyrroline N-oxide

DMPO (5,5-Dimethyl-1-pyrroline N-oxide) is a kind of water-soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance. It has a high reaction rate constant for superoxide and hydroxyl radicals and distinguishes simultaneously among a variety of important biologically generated free radicals.

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DMPO, CAS 3317-61-1

Pack Size	Availability	Price/USD
25 mg	In stock	$ 39.00
50 mg	In stock	$ 64.00
100 mg	In stock	$ 98.00
200 mg	In stock	$ 155.00
500 mg	In stock	$ 262.00

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Purity: 99.39%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	DMPO (5,5-Dimethyl-1-pyrroline N-oxide) is a kind of water-soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance. It has a high reaction rate constant for superoxide and hydroxyl radicals and distinguishes simultaneously among a variety of important biologically generated free radicals.
In vitro	In vitro, It was demonstrated in red blood cells and in hamster V79 cells that the DMPO spin adducts of ·O2? and ·OH are metabolized very rapidly so that even if formed, they may not be detected in many experiments with cells [1].
In vivo	When DMPO perfused the heart at 100 μm/L, it attenuated the development of reperfusion arrhythmias during the first 10 min of reperfusion and improved the functional recovery of the heart during reperfusion. Without treatment, 55% of hearts showed irreversible ventricular fibrillation, and this was completely prevented by DMPO, due to its function of traping·O2? and ·OH radicals [2].

Synonyms	5,5-Dimethyl-1-pyrroline N-oxide
Molecular Weight	113.16
Formula	C6H11NO
CAS No.	3317-61-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 12mg/mL (106.04 mM)

References and Literature

1. Samuni A, Samuni A, Swartz HM. The cellular-induced decay of DMPO spin adducts of .OH and .O2. Free Radic Biol Med. 1989;6(2):179-83. 2. Tosaki A1, Blasig IE, Pali T, et al. Heart protection and radical trapping by DMPO during reperfusion in isolated working rat hearts. Free Radic Biol Med. 1990; 8 (4):363-72.

Related compound libraries

This product is contained In the following compound libraries：

Bioactive Compound Library Bioactive Compounds Library Max Human Metabolite Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

DMPO 3317-61-1 Others inhibit Inhibitor 5,5-Dimethyl-1-pyrroline N-oxide inhibitor

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