keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Calcein Blue is a blue-fluorescent, short-term dye.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 30.00 | |
25 mg | In stock | $ 47.00 | |
50 mg | In stock | $ 68.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 39.00 |
Description | Calcein Blue is a blue-fluorescent, short-term dye. |
In vitro | Calcein blue AM is only weakly fluorescent (excitation/emission maxima 322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases, and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of 360/449 nm [1]. When dopamine is added to a solution of the calcein blue–Fe2 complex, dopamine–Fe2 complex is formed as the result of a ligand exchange reaction between calcein blue and dopamine which permits the fluorescence from calcein blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the calcein blue–Fe2 complex solution, which permits dopamine to be quantitatively determined [2]. |
Molecular Weight | 321.28 |
Formula | C15H15NO7 |
CAS No. | 54375-47-2 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6 mg/mL (18.68 mM), Sonication and heating are recommended.
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Calcein Blue 54375-47-2 Others inhibit iminodiacetic metallofluorochromic acid Fe2+ Inhibitor dye coumarin dopamine Fluorescent inhibitor