Shopping Cart

Your shopping cart is currently empty

AS1842856

Name: AS1842856
Brand: TargetMol
SKU: T6762
Price: 47 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T6762Cas No. 836620-48-5

AS1842856 is a Foxo1 inhibitor (IC50=30 nM) that specifically blocks the transcriptional activity of Foxo1 and reduces its activity by directly binding to activated FoxO1. AS1842856 has autophagy inhibitory activity.

AS1842856

Copy Product Info

😃Good

Purity: 99.62%

Catalog No. T6762Cas No. 836620-48-5

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$47	In Stock	In Stock
10 mg	$67	In Stock	In Stock
25 mg	$109	In Stock	In Stock
50 mg	$155	In Stock	In Stock
100 mg	$238	In Stock	In Stock
200 mg	$352	In Stock	In Stock
500 mg	$595	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.62%

Color:White to Yellow

Resource Download

COA LCMS HNMR HPLC

Product Introduction

Bioactivity

Description	AS1842856 is a Foxo1 inhibitor (IC50=30 nM) that specifically blocks the transcriptional activity of Foxo1 and reduces its activity by directly binding to activated FoxO1. AS1842856 has autophagy inhibitory activity.
Targets&IC50	FOXO1:33nM
In vitro	METHODS: Human monocytes were treated with BAY 2416964 (1 nM-10 µM), kynurenic acid (200 µM), and LPS (10 ng/mL) for 24 h. TNF-α levels were measured by ELISA. RESULTS: kynurenic acid-induced AhR activation inhibited TNF-α production in LPS-stimulated monocytes, and BAY 2416964 dose-dependently rescued this AhR-mediated inhibition. [1]
In vivo	METHODS: To assay antitumor activity in vivo, BAY 2416964 (30 mg/kg) was administered orally to NSG mice bearing B16F10-OVA tumors once daily for seven days. RESULTS: BAY 2416964 inhibited tumor growth.BAY 2416964 increased the frequency of immunostimulatory tumor-infiltrating CD8+ T cells and N cells, and decreased the frequency of immunosuppressive GR1-positive myeloid cells and CD206+M2 macrophages. [1]
Cell Research	Fao cells are serum-starved (1 h) and incubated for 30 min with either insulin or AS1842856 at the indicated concentration. Protein lysates are prepared from cells treated with either insulin or AS1842856, and relative concentration of phosphorylated Foxo1 protein is determined by Western blot analysis. (Only for Reference)

Chemical Properties

Molecular Weight	347.38
Formula	C₁₈H₂₂FN₃O₃
Cas No.	836620-48-5
Smiles	CCn1cc(C(O)=O)c(=O)c2c(N)c(F)c(NC3CCCCC3)cc12
Relative Density.	1.370 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 3.48 mg/mL (10.02 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+90% Saline: 0.1 mg/mL (0.29 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8787 mL	14.3935 mL	28.7869 mL	143.9346 mL
5 mM	0.5757 mL	2.8787 mL	5.7574 mL	28.7869 mL
10 mM	0.2879 mL	1.4393 mL	2.8787 mL	14.3935 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc