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5-Ethynyl-2'-deoxyuridine

Catalog No. T17341   CAS 61135-33-9

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor de novo DNA synthesis through click chemistry.It is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.

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5-Ethynyl-2'-deoxyuridine Chemical Structure
5-Ethynyl-2'-deoxyuridine, CAS 61135-33-9
Pack Size Availability Price/USD Quantity
50 mg In stock $ 33.00
100 mg In stock $ 59.00
200 mg In stock $ 98.00
500 mg In stock $ 182.00
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Purity: 100%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description 5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor de novo DNA synthesis through click chemistry.It is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.
In vitro PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].Like 5-bromo-2’-deoxyuridine (BrdU), EdU is incorporated into DNA during active DNA synthesis. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.Modified nucleosides, including EdU, can be cytotoxic when added to cells for prolonged periods[2].5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system[3]
Molecular Weight 252.22
Formula C11H12N2O5
CAS No. 61135-33-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 8mg/mL (31.72 mM), Sonification is recommended

DMSO: 120 mg/mL (475.77 mM), sonification is recommended.

TargetMolReferences and Literature

1. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 2. Cavanagh B L , Walker T , Norazit A , et al. Thymidine Analogues for Tracking DNA Synthesis[J]. Molecules, 2011, 16(9):7980-7993. 3. Zeng C , Pan F , Jones L A , et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system.[J]. Brain Research, 2010, 1319(none):21-32.

Related compound libraries

This product is contained In the following compound libraries:
Covalent Inhibitor Library Nucleotide Compound Library Human Metabolite Library

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Keywords

5-Ethynyl-2'-deoxyuridine 61135-33-9 Others PROTAC PROTAC Linker 5 Ethynyl 2' deoxyuridine PROTAC Linkers inhibit Inhibitor 5Ethynyl2'deoxyuridine inhibitor

 

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