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EM-12

Name: EM-12
Brand: TargetMol
SKU: T41362
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T41362Cas No. 26581-81-7

Alias Phthalimidinoglutarimide, 2-(2,6-Dioxopiperidin-3-yl)phthalimidine

2-(2,6-Dioxopiperidin-3-yl)phthalimidine (EM-12 (2-(2,6-Dioxopiperidin-3-yl)phthalimidine)) is a teratogenic Thalidomide analogue that is more active and significantly more stable against hydrolysis than Thalidomide, enhancing 1,2-dimethylhydrazine-induced rat colon adenocarcinomas [1] [2].

EM-12

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Purity: 99.34%

Catalog No. T41362Alias Phthalimidinoglutarimide, 2-(2,6-Dioxopiperidin-3-yl)phthalimidineCas No. 26581-81-7

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$40	In Stock	In Stock
25 mg	$81	In Stock	In Stock
50 mg	$118	In Stock	In Stock
100 mg	$179	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.34%

Appearance:Solid

Color:Blue

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COA LCMS HNMR

Product Introduction

EM-12 AI Summary

EM-12 displays significant bioactivity and pharmacokinetic properties across a range of assays. Notably, it inhibits angiogenesis in a rat aortic ring model, exhibiting a consistent 30% inhibition of microvessel outgrowth at concentrations of 100 uM and 200 uM in Endothelial Basal Medium with Dimethylsulfoxide. The compound demonstrates favorable distribution characteristics, with a logD value of 0.498 at 1 mg/ml under pH 7.4 conditions and good permeability in human Caco-2 cells, with an apparent permeability of 22.2 x 10^-6 cm/s and an efflux ratio close to 1 (0.99). It also has high kinetic solubility in PBS at 200 uM, with solubility reaching 451,000 nM after 30 minutes of incubation. In terms of metabolic stability, EM-12 exhibits a half-life of 1.983 hours in human plasma and greater than 3.1 hours in human liver microsomes in the presence of NADPH. The compound shows relatively low intrinsic clearance rates in both human liver microsomes and hepatocytes, indicating higher metabolic stability. Within human MOLT-4 cells, EM-12 achieves complete drug accumulation and maintains a high intracellular bioavailability of 220.0%. The compound also modulates CRBN/IKZF1, demonstrating Protac activity with a DC50 value of 192.7 nM and 69.0% maximum degradation of IKZF1 after 24 hours. Overall, EM-12 offers substantial therapeutic potential due to its robust bioactivity, favorable distribution, and metabolic profiles..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	2-(2,6-Dioxopiperidin-3-yl)phthalimidine (EM-12 (2-(2,6-Dioxopiperidin-3-yl)phthalimidine)) is a teratogenic Thalidomide analogue that is more active and significantly more stable against hydrolysis than Thalidomide, enhancing 1,2-dimethylhydrazine-induced rat colon adenocarcinomas [1] [2].
Synonyms	Phthalimidinoglutarimide, 2-(2,6-Dioxopiperidin-3-yl)phthalimidine

Chemical Properties

Molecular Weight	244.25
Formula	C₁₃H₁₂N₂O₃
Cas No.	26581-81-7
Smiles	O=C1N(CC=2C1=CC=CC2)C3C(=O)NC(=O)CC3
Relative Density.	1.2650 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 11 mg/mL (45.04 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0942 mL	20.4708 mL	40.9417 mL	204.7083 mL
5 mM	0.8188 mL	4.0942 mL	8.1883 mL	40.9417 mL
10 mM	0.4094 mL	2.0471 mL	4.0942 mL	20.4708 mL
20 mM	0.2047 mL	1.0235 mL	2.0471 mL	10.2354 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc