IL-12B Protein, Mouse, Recombinant (His)

Name: IL-12B Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03198
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: p40, interleukin 12B, Il-12p40, Il12p40, Il-12b) Copy Product Info

Synonyms: p40, interleukin 12B, Il-12p40, Il12p40, Il-12b

Catalog No. TMPY-03198 Copy Product Info

Purity: 95.3%

IL-12B Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.2 kDa and the accession number is P43432.

Pack Size	Price	USA Stock	Global Stock
5 µg	$50	-	In Stock
10 µg	$79	-	In Stock
20 µg	$127	-	In Stock
50 µg	$245	-	In Stock
100 µg	$416	7-10 days	7-10 days
200 µg	$703	7-10 days	7-10 days
500 µg	$1,410	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.3%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit IL-23 induced IL-17 secretion by mouse splenocytes. The ED50 for this effect is typically 3-20ng/mL.
Description	IL-12B Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.2 kDa and the accession number is P43432.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P43432
Construction	A DNA sequence encoding the mouse IL12B (NP_001290173.1)(Met1-Ser335) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Met 23
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	p40, interleukin 12B, Il-12p40, Il12p40, Il-12b
Research Background	Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

37.2 kDa (predicted); 47-51 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.